摘要
目的:探讨黄芪对肾间质纤维化改善的作用机制。方法:体外培养人肾间质成纤维细胞(KFB),传至3代后分为3组:对照组(常规培养),血管紧张素Ⅱ(AngⅡ)培养组(在培养液中分别加入10-8mol/L、10-7mol/L和10-6mol/L的AngⅡ),黄芪注射液干预组(分别于培养液中加入10-6mol/L的AngⅡ和10mg/L、20mg/L和40mg/L黄芪注射液)。作用48h后,各组用MTT比色法测定细胞增殖情况,用ELISA法测定细胞培养上清液中TGF-β1的水平。结果:AngⅡ可促进KFB增殖,刺激TGF-β1的分泌,且呈剂量依赖性(r分别为0.612和0.723,P<0.05);黄芪注射液可抑制AngⅡ的上述作用,且呈剂量依赖性(r分别为-0.561和-0.689,P<0.05)。结论:黄芪可以通过抑制成纤维细胞增殖和TGF-β1的分泌,延缓肾间质纤维化进展。
Aim： To explore the protective effects of astragalus membranaous on human renal interstitial fibroblasts. Methods： The human renal interstitial fibroblasts were cultured. The third generation cells were allocated into three groups： control group（ cells were cultured under normal conditions） , Ang Ⅱ groups （ cells were cultured with 10^-8 mol/L, 10^-7 mol/L,or 10^-6 mol/L Ang Ⅱ ） , Astragalus membranaous groups（ cells were cuhared with 10^-6 mol/L AngⅡ and 10 mg/L,20 mg/L, or 40 mg/L astragalus membranaous） , respectirely. After 48 h, the cellular proliferation was determined using MTT colorimetry, the levels of TGF-β1 in the supernatants were measured using ELISA. Results： AngⅡ induced the proliferation and the secretion of TGF-β1 in fibroblasts in dose dependent way, while astragalus membranaous reduced the effects of Ang Ⅱ. Conclusion： By inhibiting the proliferation and the secretion of TGF-β1 in fibroblasts, astragalus membranaous can delay the progression of renal interstitial fibrosis.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2005年第5期871-873,共3页
Journal of Zhengzhou University(Medical Sciences)
关键词
黄芪
肾脏间质成纤维细胞
细胞增殖
转化生长因子-Β1
astragalus membranaous
renal interstitial fibroblast
proliferation
transforming growth factor-β1
