摘要
目的鉴定来自新生儿病房血液培养的产超广谱β-内酰胺酶(ESBLs)肺炎克雷伯菌的β-内酰胺酶SHV基因。方法应用PCR技术对分离自新生儿病房血培养样本的5株产ESBLs肺炎克雷伯菌进行SHV基因扩增,利用ABI 377自动荧光测序仪对PCR扩增产物进行测序,并采用琼脂稀释法测定11种抗菌药物对产酶菌的最低抑菌浓度(MIC)。结果5株肺炎克雷伯菌SHV基因PCR扩增均阳性,DNA序列分析证实其中4株为SHV-12型,另外1株产一种新SHV型β-内酰胺酶,该酶与SHV-1比较显示2个氨基酸位点突变,分别为丙氨酸(Ala)134→缬氨酸(Val)和脯氨酸(Pro)269→亮氨酸(Leu),新型β-内酰胺酶经确认并命名为SHV-59(Gen-Bank注册号:AT790341)。结论本院发现一新型β-内酰胺酶SHV-59。
OBJECTIVE To determine the genotype of β-lactamases produced by ESBLs-producing Klebsiella pneu moniae isolated from blood samples of newborn department in Shenzhen People＇s Hospital. METHODS Five ESBLs-producing K. pneumoniae strains were isolated from blood samples of newborn department. PCR was used to detect genes SHV , DNA sequences were analyzed by the use of ABIPRISM 377 DNA sequencer. Minimal inhibitory concentrations （MIC） of 11 antibiotics for the isolates were determined by the agar dilution method. RESULTS Gene SHV was detected in all 5 K. pneumoniae isolates by PCR, DNA sequences showed 4 of 5 isolates were carrying gene SHV-14, the rest isolate produced a novel SHV-type β-lactamase. This enzyme differed from SHV-1 at amino acid positions, Ala 134→Val and Pro 269→Leu. It was assigned the name of SHV-59 （GenBank No. AY790341）. CONCLUSIONS A novel β-lactamase is found in our hospital.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2005年第9期965-968,共4页
Chinese Journal of Nosocomiology
