摘要
目的探讨EB病毒(Epstein-Barrvirus,EBV)感染与胃癌的关系。方法应用聚合酶链反应(PCR)-Southern技术检测185例胃癌组织和相应癌旁组织中EBVDNA,对阳性标本进一步用原位杂交试验检测EBV编码的小RNA(EBER-1),以确症EB病毒感染的存在。结果185例胃癌组织中有13例EB病毒感染,阳性率为7.03%,而相应癌旁组织中均未检测到EB病毒感染,二者有显著性差异(χ2=11.077,P<0.005)。结论EB病毒感染与胃癌的发生有一定的相关性。
Objective To investigate the relationship between Epstein-Barr virus （EBV） and gastric carcinomas.Methods The 185 specimens of gastric carcinoma tissues and 185 specimens of corres- ponding tissues adjacent to the gastric carcinoma were tested for EBV DNA genome by polymerase chain reaction （PCR）-Southern blotting.The presence of EBV was further confirmed by in-situ hybridization （ISH） in paraffin-embedded sections for EBV-encoded small RNA1 （EBER1） among the positive specimens of PCR. Results EBER1 was detected in 13（7.03%） gastric carcinomas,but absent in the corresponding adjacent tissues of gastric carcinomas. The difference between the two groups was significant（X^2=11.077,P〈0.005）. Conclusion EBV infection is associated with the pathogenesis of gastric carcinomas.
出处
《实用医药杂志》
2005年第9期788-789,共2页
Practical Journal of Medicine & Pharmacy
关键词
胃癌
EB病毒
Stomach neoplasm Epstein-Barr virus
