摘要
背景:海马组织中乙酰胆碱的代谢情况可在一定程度上反映与学习记忆相关的胆碱能系统的功能状况,并影响智能程度。目的:观察大鼠脑缺血再灌注后海马组织胆碱乙酰基转移酶活力的动态变化。设计:以实验动物为观察对象的随机对照实验研究。单位:承德医学院科研处。材料:实验于2002年在承德医学院中心实验室完成。W istar大鼠24只,体质量260～280g,雌雄各半,清洁级。方法:24只大鼠随机分为3组:①模型组。高脂血症大鼠,阻断大鼠双侧颈总动脉进行脑缺血再灌注。②假手术组。高脂血症大鼠仅做双侧颈总动脉分离术,不进行缺血再灌注。③正常对照组。不加任何处理。各组大鼠分别于术后第1,7,15d 断头取脑,用比色法测海马组织胆碱乙酰基转移酶活力。主要观察指标:大鼠脑海马组织胆碱乙酰基转移酶活力的测定。结果:24只大鼠进入结果分析,无脱失。①模型组大鼠第1天、第7天胆碱乙酰基转移酶活力较正常对照组明显降低犤模型组:(0.037±0.006)μm ol/g·s,(0.017±0.006)μm ol/g·s;正常组对照组:(0.054±0.003) μm ol/g·s,(0.058±0.006)μm ol/g·s,P <0.01犦。②模型组大鼠第7天胆碱乙酰基转移酶活力明显低于第1天,随着缺血再灌注损伤的修复,第15天胆碱乙酰基转移酶活力有所回升(0.039±0.007)μnm ol/g·s。③假手术组胆碱乙酰基转移酶活力与正常组无明显差异(P >0.05)。结论:单纯手术不会导致脑组织胆碱乙酰基转移酶的变化,脑缺血再灌注后可使海马胆碱乙酰基转移酶活力降低,使中枢胆碱能系统功能紊乱,可能是导致大鼠智能障碍的原因。
BACKGROUND： The metabolism of acetylcholine in hippocampus refleets the function of cholinergic nervous system whose function is associated with learning and memory as well as intelligence. OBJECTIVE： To observe the changes of choline acetyltransferase activity in rat hippoeampus after isehemiaeperfusion. DIg, SIGN： Randomized controlled trial based on rats. SETTING： A Science and Research Department of Chengde Medical College. MATERIALS：The trial was conducted in the Central Laboratory of Chengde Medical College in 2002 and the subjects were 24 clean grade Wistar rats in equal number of the two sexes（weighting 260-280 g）. METHODS：The 24 rats were randomly assigned into 3 groups： ①Model group：In this group the rats were made hyperlipemia and underwent bilateral carotid arteries blocking followed by reperfusion. ②Sham operation group：In this group the rats were made hyperlipemia and underwent only exposure of bilateral carotid arteries without ischemia-reperfusion. ③Normal control group：In this group the rats received no intervention.The brains after the rats decapitated were harvested on the 1^st, 7^th and 15^th day respectively for colorimetric determination of the choline acetyhransferase activity in hippoeampus. MAIN OUTCOME MEASURES：Determination of the choline acetyltransferase activity in the groups. RESULTS：None of the 24 rats was lost in the trial. ①The choline acetyltransferase activity in the model group on the 1^st and 7^th day was lower than that in the normal controls [（0.037±0.006） μmol/g-s, （0.017±0.006） μmol/g· s in model group vs （0.054±0.003） μmol/g· s, （0.058±0.006） μmol/g· s in normal control group,P 〈 0.01].② The choline acetyltransferase activity in the model group on the 7^th day was lower than that on the 1^st day.With repairing of ischemia-reporfusion injury, it recovered partially[（0.039±0.007） μmol/g· s].③ Choline acetyhrans-ferase activity in sham operation group was not different from that in normal control（P 〉 0.05）. CONCLUSION：Simple exposure of carotid arteries does not change choline acetyltransferase activity.While ischemia-reperfusion can change the choline acetyltransferase activity and cause disorders of cholinergic nervous system function,which may be the reason for rat＇s intellectual disorders.
出处
《中国临床康复》
CSCD
北大核心
2005年第24期242-243,共2页
Chinese Journal of Clinical Rehabilitation
基金
河北省科技厅资助项目(01276209D)~~
