摘要
目的:观察中药复方驱铅制剂对铅染毒大鼠记忆改善的影响,并以依地酸钙钠做标准对照。方法:实验于2001-11/12在湖南师范大学生命科学院动物实验室完成。①选用健康SD大鼠60只,随机分成6个组,每组10只。阴性对照组、阳性对照组、驱铅灵(由党参15g、鸡血藤15g等组成)3.3g/(kg·d)组、驱铅灵6.6g/(kg·d)组、驱铅灵13.2g/(kg·d)组、依地酸钙钠组。②阳性对照组、依地酸钙钠组、驱铅灵各组均用20g/L的醋酸铅溶液以10μL/g体质量的剂量灌胃,1次/d,第11天停染1d,共3周。③停止染毒后第2天,驱铅灵各组分别用相应剂型的中药复方驱铅灵10μL/g体质量灌胃,1次/d,第11天停1d,共3周。④依地酸钙钠组用5g/L依地酸钙钠溶液以10μL/g体质量的剂量(50mg/kg)腹腔注射,1周注射4d,停3d,共3周。⑤阴性对照组、阳性对照组用蒸馏水灌胃,1次/d,第11天停染1d,共3周。⑥观察驱铅灵制剂对铅染毒大鼠脑海马组织结构的改善以一氧化氮合酶阳性细胞数目的多少及阳性细胞产物平均吸光度的大小作为评价指标,对大鼠海马结构形态学损伤以一氧化氮合酶阳性产物吸光度表示。结果:纳入60只,均进入结果分析,无缺失值。①铅染毒大鼠脑齿状回阳性产物吸光度情况:驱铅灵3.3g,6.6g,13.2g/(kg·d)组与依地酸钙钠组显著大于阳性对照组犤(0.0692±0.0043),(0.0707±0.0022),(0.0645±0.0027),(0.0720±0.0037),(0.0538±0.0068)A,(F=12.383,P<0.01)犦。②铅染毒大鼠脑CA1区阳性产物吸光度变化:驱铅灵3.3g,6.6g,13.2g/(kg·d)组与依地酸钙钠组的阳性产物吸光度显著大于阳性对照组犤(0.0638±0.0033),(0.0648±0.0027),(0.0613±0.0029),(0.0655±0.0037),(0.0605±0.0026),(F=10.585,P<0.01)犦。③铅染毒大鼠脑CA3区阳性产物吸光度变化:驱铅灵3.3g,6.6g,13.2g/(kg·d)组与依地酸钙钠组在脑CA3区显著大于阳性对照组犤(0.0682±0.0044),(0.0702±0.0047),(0.0630±0.0044),(0.0733±0.0051),(0.0545±0.0027),(F=18.867,P<0.01)犦。结论:中药复方驱铅制剂低、中、高各剂量组均能改善铅染毒大鼠脑海马结构的形态学损害,改善铅致学习记忆的损害,其效果与依地酸钙钠相似,不同剂量的驱铅制剂作用效果比较无显著差别。
AIM： To observe the effects of traditional Chinese medicine compound （Quqianling decoction）on amelioration of memory in lead-exposed rats, and use antallin as controlled standard. METHODS： The experiment was done in Animal Laboratory of College of Life Science of Hunan Normal University. ①Sixty healthy SD rats were selected and divided randomly into 6 groups with 10 in each group. Negative controlled group, positive controlled group, Quqianling group 3.3 g/kg per day （composed of tangshen 15 g, suberect spatholobus stem 15g, etc.）, Quqianling group 6.6 g/kg per day, Quqianling group 13.2 g/kg per day and antallin group. ②Positive controlled group, antallin group and Quqianling group were treated with gastric perfusion of 20 g/L lead acetate solutions at the dosage of 10 μL/g body mass with once a day and at the 11^th day stopped for one day totally for three weeks. ③At the second day after stopping using narcotics, the rats in Quqianling group were treated with gastric perfusion of the corresponding type solution at the dosage of 10 μL/g Chinese compound Quqianling with once a day and at the 11^th day stopped for one day totally for 3 weeks. ④Rats in antallin group were injected in abdominal cavity with 5 g/L antallin solutions at the dosage of 10 μL/g （50 mg/kg） body mass and four days per week, stopped for three days, totally three weeks. ⑤ Negative group and positive group was treated with gastric perfusion of distilled water, once a day, the 11^th stopped for one day, totally three weeks. ⑥Effect of Quqianling on the amelioration of brain hippocampi tissue structure in lead-exposed rats was observed. Evaluation was conducted by the number of positive cells of nitricoxide synthase and average absorbance of product of positive cells. Morpholog injury of hippocampal formation in rats was expressed by absorbance of positive product of nitricoxide synthase. RESULTS： Totally 60 rats were involved in results analysis without deletion. ①Absorbance of brain dentate gyrus positive product in leadexposed rats： Absorbance of Quqianling group 3.3 g/kg per day, Quqianing group 6.6 g/kg per day, Quqianling group 13.2 g/kg per day and antallin group was higher significantly than that of positive controlled group （0.069 2±0.004 3）, （0.070 7±0.002 2）, （0.064 5±0.002 7）, （0.072 0±0.003 7）, （0.053 8±0.006 8）A （F=12.383,P 〈 0.01 ）. ②Changes of absorbance of positive product in brain CA1 area in lead-exposed rats： Absorbance of Quqianling group 3.3g/kg per day , Quqianling group 6.6g/kg per day, Quqianling group 13.2g/kg per day and antallin group was higher significantly than that of positive controlled group （0.063 8±0.003 3）, （0.064 8 ±0.00 27）, （0.061 3±0.002 9）, （0.065 5±0.003 7）, （0.060 5±0.002 6） （F=10.585, P〈 0.01）. ③Changes of absorbance of positive product in brain CA3 area in lead-exposed rats： Absorbance of Quqianling group 3.3 g/kg per day, Quqianling group 6.6 g/kg per day, Quqianling group 13.2 g/kg per day and antallin group was higher significantly than that of positive controlled group （0.068 2±0.004 4）,（0.070 2 ±0.004 7）, （0.063 0±0.004 4）, （0.073 3±0.005 1 ）, （0.054 5±0.002 7）（F=18.867, P 〈 0.01 ）. CONCLUSION： Chinese compound Quqianling decoction no matter low, middle or high dosage can improve morphology impairment of brain hippocampal formation in lead-exposed rats and the injury of learning memory induced by lead. Its effect is similar with antallin. Effects of different dosage Quqiarding decoction are insignificantly.
出处
《中国临床康复》
CAS
CSCD
北大核心
2005年第24期104-105,共2页
Chinese Journal of Clinical Rehabilitation
基金
湖南省科技厅资助项目(99ssy-2002-8)~~
