摘要
以表达新疆绵羊种布鲁氏菌外膜抗原蛋白OMP36的表达蛋白OMP2b,探索其作为诊断抗原和分子疫苗的可能性。采用PCR扩增技术,从新疆绵羊种布鲁氏菌基因组DNA中扩增出OMP2b基因片段,将该片段克隆于原核表达载体PE-28a(+),构建成重组质粒,IPTG诱导表达,SDS-PAGE检测有无蛋白的表达。结果表明,获得长约1 083bp的PCR片段,序列分析结果与已知绵羊种OMP2b同源性达89.72%;SDS-PAGE检测表达产物,在相对分子量39 ku获得了新疆绵羊种布鲁氏菌外膜蛋白OMP36的表达蛋白OMP2b基因片段,并在大肠杆菌中实现了表达。
Objective： to express OMP2b antigen protein of OMP36 from out membrane protein of Brucella ovis in XinJiang and study the probability for it as molecular bacterin and diagnose antigen. Methods ： OMP2b protein gene fragment was amplified from genomic DNA of Brucella ovis in XinJiang through PCR. The fragment was identified and cloned into prokaryotic expression vector PET-28a （ ＋ ）. Then IPTG was used to induce expression of OMP2b antigen protein. Result ： （ 1 ） An about 1 089bp length PCR product was obtained and the sequence was the same as OMP2b of B. ovis sequence reported before. （2） An expression band about 36 ku was found. Conclusion： we obtained OMP2b gene fragment and expressed successfully in Escherichia coll.
出处
《经济动物学报》
CAS
2005年第3期161-164,共4页
Journal of Economic Animal
基金
新疆兵团博士基金(兵博02)
