摘要
研究了木瓜蛋白酶、复合蛋白酶和胰蛋白酶水解小麦面筋蛋白后的酶解产物对反应的抑制作用.结果表明:抑制率与水解度相关,超过某一水解度(3种酶分别为5%、7%、4%)时,末端产物对反应的抑制率保持恒定,将抑制剂利用截留相对分子质量分别为20 000、10 000和5 000的超滤膜分离后,得到相对分子质量不同的4种组分,原始酶解产物和这4种组分对反应的抑制率分别为49.8%、4.9%、32.6%、38.1%、63.2%(木瓜蛋白酶),48.4%、4.7%、27.9%、29.1%、54.9%(复合蛋白酶)和63.1%、8.5%、28.3%、37.6%、85.7%(胰蛋白酶),SE HPLC分离后发现抑制剂(肽)的相对分子质量主要集中在5 000以下.
The three enzymes, papain, protamex and trypsin, were inhibited by wheat gluten end-products produced during enzymatic hydrolysis in a noncompetitive way. The inhibition ratio was correlated to the degree of hydrolysis（DH） and the inhibition keeped invariable when the DH exceed 5 % , 7 % and 4 % for the three proteinases respectively. The inhibitor was separated continuously by ultrafihration（UF） membranes with cut-off of 20 000, 10 000 and 5 000. The four fractions were obtained after UF. The original hydrolysates and four fractions brought out inhibition ratios of 49.8%, 4.9%, 32.6%, 38.1% and 63.2% for papain, 48.4%, 4.7%, 27.9%, 29.1% and 54.9 % for protamex, 63.1%, 8.5 %, 28.3 %, 37.6 % and 85.7 % for trypsin, respectively. The molecular size distribution analyzed by SE-HPLC indicated that the inhibitors in the peptides were less than 5 000.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2005年第4期5-8,31,共5页
Journal of Henan University of Technology：Natural Science Edition
基金
国家"十五"科技攻关课题(2001BA501A04B)
河南省高校青年骨干教师资助项目
关键词
小麦面筋
酶解
抑制剂
超滤膜
体积排阻-高效液相色谱
wheat gluten
enzymatic hydrolysis
inhibitor
ultrafihration（UF） membrane
size exclusion-high performance liquid chromatography
