期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

展示肿瘤特异性抗原表位的噬菌体在小鼠体内引起的细胞免疫变化 被引量：1

Filamentous bacteriophage displayed tumor-specific antigen epitope(MAGE-A1) induced cellular immune in mice
下载PDF
导出
摘要 以含有肿瘤特异性抗原表位的杂合型噬菌体(TSPA-M-A1)作为抗原,免疫C57BL/6J小鼠,观察免疫4周后小鼠的细胞免疫变化.结果表明:抗原TSPA-M-A1在小鼠体内能够提高T淋巴细胞对ConA刺激的反应性,使NK细胞杀伤活性明显增高,产生了特异性T淋巴细胞(CTL)反应,引起了较强的迟发型超敏反应.这些结果为肿瘤的免疫治疗提供了实验依据. The C57BL/6J mice were immunized with the purified hybrid phage, which displayed the minor - specific antigen epitope MAGE - Al161 -169 （TSPA - M - A1 ）. Tested the proliferation of T lymphocytes, NK kill rate, CTL response in the spleen by MTT, incorporating 3H- TdR and chromium- release assays. The result showed that the proliferation of T lymphocytes increased, the NK activity was enhanced obviously compared with control, elicited MAGE - A1 specific CTL responses in the spleen and delayedtype hypersensitivity（DTH）. These observations may provide a new strategy for cancer immunotherapy.
作者 房金波 赵连贵 宋金娜 高瑞娟 王丽
机构地区 东北师范大学生命科学学院 白城市教育学院
出处 《东北师大学报（自然科学版）》 CAS CSCD 北大核心 2005年第3期69-72,共4页 Journal of Northeast Normal University(Natural Science Edition)
基金 国家自然科学基金资助项目(30040014) 吉林省科学技术厅国际合作基金资助项目(20010710)
关键词 噬菌体展示 肿瘤特异性抗原 细胞免疫 CTL phage display tumor specific antigen MACE - A1 CTL
分类号 R392.12 [医药卫生—免疫学]
  • 相关文献

参考文献10

  • 1王丽.用丝状噬菌体主要外壳蛋白展示外源多肽的研究[J].科学通报,1998,43(12):1242-1246. 被引量：6
  • 2Benoit J,Van den E,Pierre van der B.T cell defined tumor antigens [J].Current Opinion in Immunology,1997(9):684-693.
  • 3Mukherji B,Chakraborty N G,Yamasaki S,et al.Induction of antigen-specific cytolytic T cells in situ in human melanoma by immunization with synthetic peptide-pulsed autologous antigen presenting cells [J].Proc Natl Acad Sci USA,1995,92(17):8 078-8 082.
  • 4Hu X,Chakraborty N G,Spom J R,et al.Enhancement of cytolytic T lymphocyte precursor frequency in melanoma patients following immunization with the MAGE-1 peptide loaded antigen presenting cell-based vaccine [J].Cancer Res,1996,56(11):2 479-2 483.
  • 5Pierre G C,Vaios K,Didier C,et al.A monoclonal cytolytic T-lymphocyte response observed in a melanoma patient vaccinated with a tumor-specific antigenic peptide encoded by gene MAGE-3 [J].Proc Natl Acad Sci USA,2001,98(18):10 290-10 295.
  • 6Pierre G Coulie,Pierre van der Bruggen.T-cell responses of vaccinated cancer patients [J].Current Opinion in Immunology,2003,15:1-7.
  • 7Rong Fu Wang.The role of MHC classII-restricted tumor antigens and CD 4+ T cells in antitumor immunity [J].TRENDS in Immunology,2001,22:269-276.
  • 8Lorenzo Moretta,Cristina Bottino,Claudia Cantoni,et al.Human natural killer cell function and receptors [J].Current Opinion in Pharmacology,2001(1):387-391.
  • 9Ying Wan,Yuzhang Wu,Jiang Bian,et al. Induction of hepatitis B virus-specific cytotoxic T lymphocytes response in vivo by filamentous phage display vaccine [J].Vaccine,2001,19:2 918-2 923.
  • 10Di Marzo Veronese F,Willis A E,Boyer-Thompson C,et al.Structural mimicry and enhanced immunogenicity of peptide epitopes display on filamentous bacteriophage.The V3 loop of HIV-1 gp120 [J].J Mol Biol,1994,243:167-172.

二级参考文献1

  • 1王林，生物工程学报，1996年，12卷，3期，235页

共引文献5

同被引文献6

  • 1STOJIKOVIC S S, REINHAST J, CATT K J. Gonadotropin releasing hormone receptors: structure and singnal traosduction pathways[J]. Endocr Rev, 1994,15(9) :462 - 469.
  • 2SCHALLY A V, SRKALOVIC G, SZENDE B, et al. Antitumor effects of analogs of LH- RH and somatostain: experimental and dinical studies[J]. J Steroid Biochem Mol Biol, 1990,37(10) : 1 061 - 1 067.
  • 3FRANKEL A E, KRITMAN R J, SAUSVILLE E A. Targeted toxins[J ]. Clin Cancer, Res, 2000(6):326- 334.
  • 4KREITMAN R J, PASTAN I. Immunotoxins for targeted cancer therapy[J]. Adv Drug Delive Rev, 1998,31:53- 88.
  • 5WAWZRYNCZAK E J. Systemic immunotoxin therapy of cancer:advances and prospected[J]. Br J Cancer, 1991,64(5) :624 - 630.
  • 6GILL D M, PAPPENHEIMER A M. Structure activity relationships in diphtheria toxin[J] .J Biol Chem, 1971,346:1 492 - 1 495.

引证文献1

二级引证文献3

东北师大学报（自然科学版）
2005年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部