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用两种相似多糖类手性固定相拆分阿托品外消旋对映体的比较 被引量：2

Comparison of Chiral Separation of Atropine Racemate with Two Similar Polysac-charide-based Chiral Stationary Phases
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摘要 分别涂敷纤维素-三(3,5-二甲基苯基氨基甲酸酯)(CDMPC)与直链淀粉-三(3,5-二甲基苯基氨基甲酸酯)(ADMPC)于自制的球形氨丙基硅胶上,制备了两种多糖类手性固定相。用高效液相色谱法(HPLC)在正相条件下,用两种固定相直接拆分了阿托品(atropine)外消旋体。在正己烷中加入了不同的醇类改性剂对阿托品进行拆分,并优化了流动相中醇类改性剂的比例。结果发现,阿托品在CDMPC固定相上可以得到基线拆分,而在ADMPC固定相上只能得到部分拆分。 Two polysaccharide-based chiral stationary phase（CSP） were prepared by coating amylase- tfis （3,5-dimethylphenyl carbamate） （ADMPC） and cellulose - tris （3,5-dimethylphenyl carbamate） （CDMPC） onto aminopropylated spherical silica gel, respectively. Atropine racemate was directly resolved on ADMPC- CSP and CDMPC - CSP by normal-phase high performance liquid chromatography （HPLC） using different alcoholic modifiers. The result indicated that only a partial separation of atropine racemate could be obtained with ADMPC - CSP, whereas a baseline separation of atropine racemate could be achieved with CDMPC - CSP.
作者 翟宗德 罗兴平 李欣 陈立仁 师彦平
机构地区 中国科学院兰州化学物理研究所
出处 《分析测试学报》 CAS CSCD 北大核心 2005年第5期89-91,共3页 Journal of Instrumental Analysis
基金 中国科学院百人计划资助课题
关键词 手性拆分 阿托品 高效液相色谱 多糖类手性固定相 Enantioseparation Atropine HPLC Polysaccharide-based chiral stationary phase
分类号 O657.72 [理学—分析化学] R97 [医药卫生—药品]
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参考文献9

  • 1GILMAN A G, GOODMAN L S. The Pharmacological Basis of Therapeutics[M]. Pergamon: Oxford, 1980. 120- 12l.
  • 2ARMSTRONG D W, HANS M. [J]. Analytical Biochemistry,. 1987, 167:261 - 264.
  • 3NG S C, CHEN L, ZHANG L F, et al. [J]. Tetrahedron Letters, 2002, 43:677 - 681.
  • 4TAHARA S, OKAYAMA A, KITADA Y. [J]. J Chromatogr, A, 1999, 848: 465- 471.
  • 5MATEUS L, CHERKAOUI S, CHRISTEN P. [J]. J Chromatogr, A, 2000, 868:285 - 294.
  • 6HUISDEN R E, KRAAK J C, POPPE H. [J]. J Chromatogr, 1990, 508(2) : 289 - 299.
  • 7OKAMOTO Y, KAWASHIMA M, HATADA K. [J]. J Chromatogr, 1986, 363(2) : 173- 186.
  • 8WAINER I W, STIFFIN R M. [J]. J Chromatogr, 1987, 411: 139- 151.
  • 9PERSSON B, ANDERSSON S. [J]. J Chronlatogr, A, 2001. 906: 195- 203.

同被引文献24

  • 1杨凌,冉桂梅,张才华,刘可,郭兴杰.α_1-酸性糖蛋白柱拆分马来酸曲美布汀对映体[J].分析化学,2005,33(5):668-670. 被引量：4
  • 2BRETON D, BURET D, CLAIR P, et al. Chiral separation of atropine by high-performance liquid chromatog- raphy[ J]. J Chromatogr A,2005,1088 : 104 - 109.
  • 3DANUTA S, DONALD E M, NAOMI G, et al. HPLC-atmospheric pressure chemical ionization mass spectro- metric method for enantioselective determination of R, S-propranolol and R, S-hyoscyamine in human plasma [ J]. J Chromatogr B ,2007,859:213 - 221.
  • 4LIDIA M, SAMIR C, PHILIPPE C, et al. Enantioseparation of atropine by capillary electrophoresis using sulfatedβ-cyclodextrin: application to a plant extract[ J ]. J Chromatogr A,2000,868:285 - 294.
  • 5SYUN-ICHIRO T, AKIKO O, YOSHIMI K, et al. Enantiomeric separation of atropine in Scopolia extract and Scopolia Rhizome by capillary electrophoresis using cyclodextrins as chiral selectors [ J ]. J Chromatogr A, 1999,848:465 - 471.
  • 6JORGEN H, ANDERS G. Optimization of the separation of enantiomers of basic drugs retention mechanisms and dynamic modification of the chiral bonding properties on an α1-acid glycoprotein column [ J ]. J Chromatogr A, 1995,694:57 - 69.
  • 7Palkin S, Watkins HR. The stability of atropine and hyoscyamine dur- ing process of analysis[J]. JAm Pharm Assoc, 1927, 16(1) : 21-30.
  • 8Grynkiewicz G, Gadzikowska M. ChemInform Abstract: Tropan Alka- loids as Medicinally Useful Natural Products and Their Synthetic Deriv- atives as New Drugs[ J]. Cheminform, 2008, 60(4): 439-463.
  • 9John H, Eyer F, Zilker T, et al. High-performance liquid-chromato- graphic tandem-mass spectrometric methods for atropinesterase-media- ted enantioselective and ehiral determination of R-and S-hyoscyamine in plasma[J]. Analytica Chimica Acta, 2010, 680(s 1-2) : 32-40.
  • 10John H, Mikler J, Worek F, et at. Application of an enantioselective LC-ESI MS/MS procedure to determine R - and S -hyoscyamine fol- lowing intravenous atropine administration in swine[J]. Drug Testing & Analysis, 2012, 4(3-4) : 194-198.

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分析测试学报
2005年 第5期

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