摘要
目的探讨维生素C对人宫颈癌HeLa细胞生长和凋亡的影响,并初步探讨其机制。方法用MTT比色法检测细胞生长抑制作用,流式细胞术测定各组细胞周期分布和凋亡率,以间接免疫荧光法流式细胞术检测相关蛋白的表达,以TRAP-PCR-ELISA法测定端粒酶活性。结果维生素C能以浓度依赖和时间依赖方式阻滞HeLa细胞于G0/G1期,抑制其生长并诱导凋亡。同时HPV18 E6、Bc l-2蛋白表达下调,wtp53、Bax蛋白表达上调,端粒酶活性降低。结论维生素C可抑制HeLa细胞生长,使其细胞周期阻滞于G0/G1期,并诱导其凋亡;其原因在于病毒癌基因E6表达的降低,以及由此而引起的端粒酶活性的降低和细胞内蛋白表达水平的改变,包括HPV18 E6、Bc l-2蛋白表达的下调,wtp53、Bax蛋白表达的上调。
Aim To study the effect of Vitamin C on growth and apoptosis of human cervical cancer cell line HeLa and its mechanisms. Methods HeLa cell line was treated with VitC. Cell proliferation was evaluated with MTT assay. The distribution of cell cycle, the rate of apoptosis and the expression of HPV18 E6,p53,Bax and Bcl-2 protein of the heLa cells, were observed using flow cytometer respectively. TRAP-PCR-ELISA assay was used to evaluate telomerase activity. Results Vitamin C arrested the HeLa cell line in G0/G1 phase in a dose and time-dependent manner, and significantly inhibited HeLa cells growth and increased the rate of apoptosis. Expression of HPV18 E6 and Bcl-2 proteins, was down regulated expression of p53 and Bax proteins was upregulated, and the telomerase activity was reduced. Conclusion Vitamin C can significantly inhibit HeLa cells growth and proliferation, arrest them in G0/G1 phase and induce their apoptosis. The reason of these changes is the decrease of E6 gene＇ s expression, and the succedent changes of proteins＇ expression in cancer cells,including the downregulated expression of HPV18 E6 and Bcl-2 proteins, upregulated expression of p53 and Bax proteins and reduced telomerae activity.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第9期1096-1099,共4页
Chinese Pharmacological Bulletin
