摘要
目的观察葛根芩连汤各配伍对菌痢模型药效,探讨其配伍规律。方法兔盲肠分流术后直接结肠内接种,制作菌痢兔模型。按正交设计方法,观察葛根芩连汤各配伍组合对菌痢模型的肠内容物细菌计数及肠组织内细菌计数,结肠炎指数的影响。结果葛根芩连汤各配伍对结肠内细菌计数及结肠组织内细菌计数,以黄连、黄芩作用为主,黄连与黄芩相互拮抗,其最佳组合为单味黄连,结肠炎指数为炙甘草。结论在葛根芩连汤各种配伍对实验性细菌性痢疾的作用上,对细菌的影响,以黄连为优;对病理学损害的改善,以炙甘草为优。
Objective To investigate the relations between different compositions of Gegenqinlian decoction and their effects on experimental shigellosis in rabbits to explore the composition protocol of Gegenqinlian decoction. Methods Rabbit models of intestinal invasion induced by Shigella flexneri was established by directly inoculating the bacterium in the colon after cecum bypass operation. The effects of different compositions of Gegenqinlian decoction on the indexes of colitis and the bacterial count of Shigella in the colon content and colon tissue were investigated by orthogonal design. Results According to the bacterial count of Shigella in the colon, Coptis chinensis franch and ScuteUaria baicalensis Georgi are the main effective drugs, but they antagonized each other, so that the best combination protocol was Coptis chinensis Franch only. Conclusion Among the components of Gegenqinlian decoction, Coptis chinensis franch has the strongest pharmcological effect against bacteria and Glycyrrhina wralensis fisch is the most effective to ameliorate pathological lesions.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第9期1132-1134,共3页
Journal of First Military Medical University
基金
国家自然科学基金(39970886)
广东省自然科学基金(001070)~~
关键词
葛根芩连汤
配伍规律
正交设计
细菌性痢疾
Gegenqinlian decoction
composition principle
orthogonal design
shigellosis
