摘要
The effects of anthocyanin from Mah,a sylvestris on plasma lipids and free radical were investigated by Reagent Kit method and in vitro assay. High tat model was set up with albino rats that were fed with different dosages of anthocyanin from Malva sylvestris 10.03 g·d^-1, 0.04 g·d ^-1 and 0.05 g·d^-1). The results showed that the total cholesterol was decreased by 19.7% at an anthocyanin of 0.04 g. d and triglyceride was decreased by 34.4% at an anthocyanin of 0.05 g·d^-1. In vitro assay, some indexes of anthocyanin were measured including the capability of scavenging free radical, reducing force and the capability of anti-lipid peroxidation by orthophenanthroline Fe^+2 oxidation-reduction method. The results indicated that the clearance rate of free radical reached to 43.46% when the content of anthocyanin was 0.20 mg·mL^-1 and the inhibition ratio of lipid peroxidation reached 18.82% when the content was 0.5 mg·mL^-1. Therefore anthocyanin is a kind of natural and effective free radical remover and antioxidant and can prevent from the formation of the thrombus and reduced the emergence of the cardiovascular disease.
从血浆类脂化合物和自由的基上的 Malva sylvestris 的 anthocyanin 的效果被试剂工具包方法和试管内试金调查。高胖的模型带着从 Malva sylvesiris 用 anthocyanin 的不同剂量被喂的白化体老鼠被建立(0.03 g · d ^(-1), 0.04 g · d^(-1) 和 0.05 g · d^(-1)) 。结果证明全部的胆固醇被 19.7% 在 0.04 g · d~ 的 anthocyanin 减少(-1) 和 triglyceride 被 34.4% 在 0.05 g · d~ 的 anthocyanin 减少(-1) 。试管内试金, anthocyanin 的一些索引包括清除的能力被测量自由激进分子,减少的力量和由 orthophenanthroline Fe^(+2 ) 的 anti-lipidperoxidation 的能力氧化减小方法。结果显示清理当 anthocyanin 的内容是 0.20 mg · m L~ 时,联系到 43.46% 的自由激进分子评价(当内容是 0.5 mg · m L~ 时, -1) 和类脂化合物的抑制比率每氧化到达了 18.82%(-1) 。因此 anthocyanin 是一种天赋和有效免费激进的搬运工和抗氧化剂并且能阻止血栓的形成并且减少心血管的疾病的出现。