摘要
以沙棘叶为原料,用正交试验法优化了沙棘总黄酮(TFH)的超声波提取工艺,考察了超声波作用时间、超声波温度、液料比和提取次数4个因素对沙棘总黄酮提取率的影响,确立了沙棘总黄酮的优化超声波提取工艺条件为:提取溶剂85%乙醇,液料比25∶1(mL∶g,下同),超声波温度46℃,超声波作用时间25min,提取1次。将该法与索氏提取进行了对比。结果表明,超声波提取沙棘总黄酮的提取率为1.72%,与索氏提取的提取率相当,但超声波提取所用提取时间短,提取溶剂用量少,因此超声波法具有明显的优势。同时对超声波提取进行了数学模拟,模拟值与试验结果吻合良好。
Ultrasonic extraction of flavonoids in leaves of Hippophae rhamnoides Linn. （ TFH ） was optimized by an orthogonal experiment. The effects of ultrasonic treatment time, temperature, ratio of liquor to material and extraction times on the extraction yield of TFH were investigated. The optimized ultrasonic extraction was determined as follows ： ratio of liquor to material 25 mL/g at 46 ℃ for 25 min with 85 % ethanol in a single ultrasonic extraction process. A comparison was conducted between ultrasonic and Soxhlet extraction. The extraction yield of TFH is 1.72 % by ultrasonic extraction, which is almost the same as by Soxhlet extraction. Ultrasonic extraction is faster and needs less solvent, which are superior to Soxhlet extraction. Mathematical simulation values based on ultrasonic extraction are consistent with experimental values.
出处
《林产化学与工业》
EI
CAS
CSCD
2005年第3期85-88,共4页
Chemistry and Industry of Forest Products
基金
科技部中德政府间合作资助项目(CHN03/028)
科技部科研条件升级改造资助项目(JG-2004-09)
关键词
沙棘叶
总黄酮
超声波提取
leaves of Hippophae rhamnoides Linn,
flavonoids
ultrasonic extraction
