摘要
猪链球菌Ⅱ型是导致许多国家猪链球菌病的主要病原,自90年代晚期江苏发生猪链球菌Ⅱ型感染 以来已成为我国引起人畜共患病的一种重要的新病原菌。最近在四川省部分地区发生了不明原因的猪源人畜 共患病,且具有较高的死亡率。我们从病死猪的病料中成功分离到三株猪链球菌分离株,经革兰氏染色、生化试 验、血清凝集试验和PCR鉴定,最终证实为猪链球菌Ⅱ型。通过对其毒力因子进行鉴定,结果发现MRP和EF 均为阳性。进一步的药敏试验证实:分离菌株对氨苄青霉素、先锋霉素Ⅵ、羧苄青霉素、复方新诺明、头孢肤肟 等抗菌药高度敏感。
Recently, an unknown infection with high mortality from pig to human occurred in Sichuan province and the epidemiological investigation suggested that it might be a zoonotic infection of Streptococcus suis. Bacteria isolation was carried on blood nutrient agar from which the suspected colonies were then selected and tested by agglutination and PCR for Streptococcus suis type Ⅱ. Three bacterial isolates from clinical samples were diagnosed as Streptococcus suis type Ⅱ based on Gram staining, biochemical character, agglutination and PCR test. PCR for virulent factor MRP （muramidase-released protein） and EF （extracellular factor） genes of Streptococcus suis type Ⅱ were all positive in the 3 isolates. Drug sensitivity tests revealed that all the isolates were highly sensitivity to ampicillin, cephalothins Ⅳ, carbenicillin, compound sulfamethoxazole and cefuroxime.
出处
《中国动物检疫》
CAS
北大核心
2005年第10期20-22,共3页
China Animal Health Inspection
