摘要
目的:探讨尿液中粘多糖含量的变化能否作为痰证的辅助实验诊断指标。方法:提取痰证、阴虚证患者及正常人各10例尿液中粘多糖,以醋酸纤维素薄膜电泳法、阿利新蓝染色定量法分析测定粘多糖种类和含量。结果:尿液中提取的粘多糖分为透明质酸和硫酸软骨素两种,总含量呈痰证组>正常组>阴虚证组变化。结论:通过进一步扩大样本的研究,尿液中粘多糖含量测定可望作为痰证的辅助实验诊断指标,并为体内粘多糖的变化与痰证之间的关系研究提供前期实验基础。
Object：To explore whether the change of the content of glycosaminoglycan（GAG） in urine can be as the assistant index for diagnosis of phlegm syndrome. Methods：GAG were taken from urine in 10 patients of phlegm syndrome, 10 patients of yin asthenia and 10 health people, Cellulose acetate membrane electrophoresis and alcian blue dyeing were used to analyze the kinds and content. Results：GAG in urine were divided into HA and CS. Among three groups, the phlegm syndrome group was the highest, the health group was the second, and the yin - dificence syndrome group lowest. Conclusion：With the further study, the content of GAG in urine can be the assistant index for diagnosis of phlegm syndrome, which providing the basis to exploring the relation between the pathological changes of GAG and phlegm syndrome.
出处
《中医药导报》
2005年第9期6-7,9,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
广州中医药大学基础医学院院长青年创新基金(BMC002)
关键词
痰证
粘多糖
电泳分析
含量测定
Syndrome of phglem
Mucoitin
Electrophoretic analysis
Assaying
