摘要
为了研制肾综合征出血热减毒活疫苗,研究病毒毒力,改进了汉坦病毒感染环磷酰胺处理地鼠动物模型,即检测地鼠感染病毒后出现的类似于人发病过程的四期经过。利用该动物模型从汉坦病毒(Ⅰ型)A9毒株中,采用空斑选育法筛选,获得1株对乳小鼠丧失脑内致死力及对环磷酰胺处理地鼠丧失致病力的弱毒株。弱毒株感染乳小鼠及环磷酰胺处理地鼠后,在肝、脾、肾、肺及脑中呈"一过性"繁殖,用免疫荧光法检测不到明显的病毒抗原,但采用逆转录-聚合酶链反应可检测到特异性病毒核酸。
For developing attenuated vaccine against hemorrhagic lever with renal syndrome(HFRS),the gold- en hamsters used as a model immuno-suppressed with cyclophosphamidum(CY)had been modified by monitering four phases of clinical course(ferbril phase,oliguric phase,diuretic phase and convalescent phase)of the disease in CY-treated hamsters infected with Hantaan virus.An attenuated virus clone was isolated from the original virulent Hantaan virus,A9 strain,with plaque cloning method,which had lost its pathogenicity to the suckling mouse mod- el and the CY-treated hamster model.Significant differences in virulence in vivo between the cloned attenuated vi- rus clone(A39s)and the cloned virulent one(A9v)were found as follows:the cloned attenuated virus showed very low infectivity,presented'transient propagation'and the virus antigen was not detectable in the liver,spleen,lung, kidney and brain of the suckling mice and the CY-treated hamsters infected with this virus,but the specific viral RNA was detectable with the method of reverse transcription and polymerase chain reaction(RT-PCR).
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1996年第1期1-5,共5页
Chinese Journal of Experimental and Clinical Virology
