摘要
采用毛细管电泳技术研究了重组促红细胞生长素(rhEPO)的分离问题。用N,N-二甲基六烷基溴化铵(6,6-ionene)涂层的毛细管测定了rhEPO中唾液酸的微多相性,同时采用毛细管电泳-质谱(CE-M S)联用技术在22m in内鉴定了rhEPO20段胰酶消化肽中的11段。该方法简单快捷,重现性好,可用于蛋白质一级结构的测定。
The microheterogeneity of recombinant human erythropoietin （rhEPO） was analyzed by capillary electrophoresis （CE） using a capillary coated with 6,6-ionene. The applicability of a volatile electrolyte for fast analysis of tryptic fragments of rhEPO with online CE-electrospray ionization-mass spectrometry （ESI-MS） was investigated, resulting in a reproducible separation of eleven rhEPO tryptic fragments within 22 rain under the following conditions ： running buffer 300 mmol/L acetic acid-ammonium acetate （HAc-NH4Ac） , pH 4.80, separation voltage - 15 kV and capillary temperature 25 ℃. The proposed method is rapid and effective, and can be used for the structural analysis of related proteins.
出处
《色谱》
CAS
CSCD
北大核心
2005年第5期487-491,共5页
Chinese Journal of Chromatography
基金
国家自然科学基金重点项目(No.20335010)
关键词
毛细管电泳
毛细管电泳-质谱联用
N
N-二甲基六烷基溴化铵涂层
重组促红细胞生长素
酶解产物
微多相性
肽图
capillary electrophoresis
capillary electrophoresis coupled with electrospray ionization-mass spectrometry
6,6-ionene coating
recombinant human erthropoietin
tryptic digest
microheterogeneity
tryptic mapping
