摘要
应用环境低温复制肉鸡肺动脉高压模型,观察肺细小动脉蛋白激酶Cα(PKCα)的表达变化,并探讨PKCα与肺血管重构的关系。160羽艾维茵-2000商品代肉鸡于14d时随机等分为常温对照组(NT组)和低温组(LT组)。自14d起,LT组舍内温度从28℃以每天1～2℃的速度下降,至21d降至14～12℃,并维持到试验结束;NT组仍继续按常规饲养(21d起舍温控制在20℃)。记录肺动脉高压综合征(PHS)发病率,并分别于24、32、39、45d从各组随机抽样,测定右心室/全心室质量比(RV/TV)、红细胞压积(PCV)、血红蛋白(Hb)、肺细小动脉管壁面积/管总面积(WA/TA)和平均中膜厚度(mMTPA);采用免疫组化方法标记PKCα,并用病理图像分析软件检测肺细小动脉光密度值(OD),以OD值代表PKCα的表达。结果表明,LT组肉鸡PHS发病率显著升高(P<0.05);RV/TV值在45d时显著升高(P<0.05);PCV和Hb值在32d后显著升高(P<0.05);血管mMTPA值和WA/TA值在各日龄段均显著升高(P<0.05);肺细小动脉OD值升高,在39d时差异显著(P<0.05),且OD值与mMTPA和WA/TA值呈正相关。上述结果表明:在肉鸡肺动脉高压发生过程中,肺小动脉PKCα表达上调,并可能促进了肺血管重构的形成。
The present study was conducted to examine the expression of protein kinase Cα （PKCα） in pulmonary arterioles in broilers with pulmonary hypertension induced by cold temperature exposure and the relationship between PKCα and pulmonary vascular remodeling. One hundred and sixty Avian-2000 broilers were divided equally into a normal temperature control group （NT） and a low temperature group （LT） at 14-day-old of age. The group LT was subjected to low temperatures by lowering 1-2℃ per day from 28 ℃ （day 14） down to 14-12 ℃ （day 21）, and remained constant until the end of the experiment, while the group NT was still reared at normal temperatures （house temperature was 20 ℃ from day 21 on）. PHS incidence was recorded. Ratio of fight/total ventricle （RV/TV）, packed cell volume （PCV） and hemoglobin （Hb） were measured at 24, 32, 39 and 45-day-old of age, respectively. Vessel wall area to vessel total area （WA/TA）, mean media thickness in pulmonary arterioles （mMTPA） were determined using computer-image analytic software, and expression of PKCα in the pulmonary arterioles labeled by immunohistochemistry and quantified by measuring optical density （OD） using computer-image analytic software. PHS incidence amounted to 3.75% and 12.5% in groups NT and LT, respectively （P 〈 0.05）. PCV in the group LT was elevated after day 32 （P 〈 0.05）, and RV/TV ratio increased on day 45 （P 〈 0.05）. Both the WA/TA and mMTPA of broilers in the group LT were significantly elevated （P 〈 0.05）. There were no differences in OD value between the two groups before day 32 （P 〉 0.05）. However, one week later （at day 39 of age）, the OD value of broilers in the group LT was significantly increased （P 〈 0.05）. The increased OD values were positively correlated with mMTPA and WA/TA. These results demonstrated that PKCα expression was increased in broilers with pulmonary hypertension, and increased expression of PKCα might enhance pulmonary vascular remodeling.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第9期1917-1922,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金(30371061)
江苏省"九五"农业重点攻关项目(BE964888)
