摘要
酶解玉米蛋白对生产高营养且易于吸收、高附加值的具有生物学功能特性的生物产品具有重要意义。本文通过AlcalaseAF2.4L和风味蛋白酶在适当条件下酶解玉米蛋白,酶解2h,水解度可达31%,短肽得率超过70%以上。经SephadexTMG-25进行分离,对其分离后的各组分进行抗氧化活性的研究,经测定,玉米短肽在浓度为1mg/ml时对邻苯三酚(PR)的自氧化抑制率达43.9%,相当于5U/mlSOD的抗氧化活性。
It is significant to make full use of corn gluten meal(CGM) with enzymolysis in that it contributes to produce biological active products with full nutrition, easy absorption and high added value. In this paper, CGM was hydrolyzed by Alcalase AF 2.4L and Flavourzyme under the optimum condition. The results showed the degree of hydrolysis was up to 31% in two hours as well as the yield of oligopeptides was 70%. In addition, oligopeptides were separated by Sephadex^TM G-25 and antioxidative activity of CGM was examined. The research indicated the inhibition rate was up to 43.9% when it's concentration was 1mg/ml as same as that of 5U/ml SOD.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第9期33-35,共3页
Food Science
基金
国家重点星火计划项目(2001EA610021)
关键词
玉米短肽
抗氧化性
水解度
corn gluten meal(CGM)
antioxidatine activity
hydrolysis degree