摘要
在7L发酵罐中考察不同发酵温度23℃～37℃下对重组大肠杆菌E. coli ZYL-2产番茄红素的影响,33℃时发酵开始至9 h细胞比生长速率高于其他温度,且最早获得最大细胞生物量,发酵9 h后,28℃番茄红素比合成速率高于其他温度,合成期延长.基于上述结论,两步温度控制策略确定在发酵前9 h温度为33℃,9 h后降低至28℃,可获得最大番茄红素产量605.25 μgL-1和生产强度28.82μg L-1h-1,番茄红素发酵水平高于单一温度.
Batch fermentation of lycopene by recombinant E. coli ZYL-2 at various temperatures ranging between 23℃～ 37℃ were studied in 7L fermentor. At 33℃, cell specific growth rate in earlier stage of culture was higher, and the time obtained maximum cell dry weight was shorter at other temperature, while after 9 h, lycopene specific production rate was higher at 28℃. Based on these results, a two-stage temperature control strategy was developed in which 33℃C was used for fermentation for the first 9 h and the temperature was switched to 28℃ after 9h. Using this temperature-shift strategy, the maximal lycopene content and productivity reached 605.25μg L^-1 and 28.82μg L^-1h^-1. The lycopene fermentation level obtained by the strategy was higher than those in single temperature-control experiments.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第5期19-23,共5页
Microbiology China
关键词
重组大肠杆菌
番茄红素
分批发酵
温度控制策略
Recombinant E. coil, Lycopene, Batch fermentation, Strategy of temperature control
