摘要
CTAB法提取灵芝菌丝体总DNA,再以核糖体DNA转录间隔区(ITSⅡ、IGRⅠ-PCR RFLP)结合随机引物扩增多态性(RAPD)标记分析30个灵芝菌株间的亲缘关系。结果表明,ITSⅡI、GRⅠ-PCR RFLP产生了47条带,其中42条显示出多态性,通过聚类分析将30个菌株分成七大类,包括紫芝、黑灵芝、树舌灵芝、薄树灵芝四大类,以及另外三个无法确定具体归属的类群,但没能将赤芝与松杉灵芝区分开。RAPD从247个随机引物中筛选出8个随机引物,共检测到69个多态性位点并全部显示出多态性,通过聚类分析将供试的30个灵芝菌株全部区分开。
Total DNA of Ganoderma mycelium was extracted by the method of CTAB, and genetic relationships among thirty Ganoderma strains were analyzed based on ITS Ⅱ、 IGR Ⅰ -PCR RFLP and RAPD. The results showedthat 47 bands were obtained based on ITS Ⅱ, IGR Ⅰ -PCR RFLP, and 42 bands of them were polymorphic.Thirty strains were divided into seven species： Ganoderma sinense, Ganoderma atrum, Ganoderma applanatum, Ganaderma capense （Loyd） Teng and other three groups （their species are still unknown） through dendrograrn aralysis. Only 8 primers produced stable results among 247 random primers by RAPD. Total 69 loci were obtained by using 8 primers and all of them were polymorphie. Based on these 69 loci, thirty Ganodo-ma strains were differentiated.
出处
《食用菌学报》
2005年第3期7-13,共7页
Acta Edulis Fungi
基金
绿谷集团有限公司灵芝专项科研资金全额资助项目"灵芝菌种DNA鉴别"(项目编号:2003SHGV01)
福建省星火计划项目"福建食用菌集聚产业科技服务体系建设示范--食用菌种质资源库建设
分类与认定前期技术研究"(项目编号:2003EAT20021)的部分研究内容
关键词
灵芝
核糖体DNA转录间隔区
随机引物扩增多态性
Ganoderma
Internal transcribed spacer（ITS）
Rondom amplified polymorphic DNA（RAPD）
