摘要
目的:研究人非小细胞肺癌(NSCLC)细胞对常用抗肿瘤药物的耐药情况及其与两种耐药因子p53抗体和GST-π表达的关系。方法:对58例NSCLC手术标本进行原代细胞培养,然后以MTT法检测其对顺铂(DDP)、长春新碱(VCR)、足叶乙甙(VP16)、羟基喜树碱(HCPT)、阿霉素(ADM)及环磷酰胺(CTX)6种临床常用化疗药物的敏感性;同时以酶联免疫吸附测定法(ELISA)检测患者血清中p53抗体及GST-π两种耐药因子表达情况。结果:6种药物的有效率(即化疗药物对肿瘤细胞的活性抑制率)分别为DDP 41.4%、VCR 24.1%、VP16 22.4%、HCPT 20.7%、ADM 17.2%及CTX 17.2%;p53抗体及GST-π表达情况不同,其表现出不同耐药程度。结论:以MTT法检测NSCLC的药物敏感性,尤其对p53抗体及GST-π表达情况的检测,对于指导肺癌化疗是可行而且必要的。
Objective To study the drug sensitivity of six kinds of chemotherapeutic drugs for human non-small cell lung cancer （NSCLC）, and their relationships with the expressions of two drug-resistance factors p53 antibody and GST-π. Methods Fifty-eight NSCLC specimens obtained by operation were used for primary culture. Then the sensitivities of the cells to six kinds of chemotherapeutic drugs were detected by MTT assay. The serum GST-π and p53 antibody in the patients were tested by ELISA at the same time. Results The effective rates of the six kinds of drugs were Cisplatin （DDP） 41.4%, Vicristine （VCR） 24.1%, Etoposide （VP16） 22.4%, Hydroxylcamptothecin （HCPT） 20. 7%, Adriamycin （ADM） 17. 2 %, Cyclophosphamide （CTX） 17.2 %, respectively. The patients had different multi-drug resistance when they had different positive expressions of p53 antibody and GST-π. Conclusion It is possible and necessary to test the drug sensitivity of lung cancer specimens obtained from operation by MTT method, and the results are very useful for the clinic chemotherapy after operation, especially when p53 antibody and/or GST-π positively express in the tissues.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期795-798,共4页
Journal of Jilin University：Medicine Edition
基金
中-加大学合作项目(SULCP010#)
