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二温式多重PCR检测对虾白斑综合征病毒和桃拉病毒的研究 被引量：4

STUDY ON THE DETECTION OF WHITE SPOT SYNDROME VIRUS AND TAURA SYNDROME VIRUS BY MULTIPLEX PCR
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摘要 对虾白斑综合征(WSS)和桃拉病(TS)是两种严重危害当前对虾养殖业的对虾病毒性疾病.目前对虾病毒病的诊断手段主要包括病理学和生物学方法、免疫学方法、分子杂交方法及PCR方法等[1-4].其中PCR方法是这些方法中特异性最强、敏感最高的病原检测手段,在国外已被广泛应用于对虾病毒病的检测和诊断.多重PCR是一种特殊PCR形式,其最突出特点,即一次PCR反应,就可同时检测、鉴别出多种病原体,在临床混合感染的鉴别诊断上具有其独特优势和很高的实用价值[5,6].
作者 谢芝勋 庞耀珊 邓显文 唐小飞 刘加波
机构地区 广西壮族自治区兽医研究所
出处 《水生生物学报》 CAS CSCD 北大核心 2005年第5期595-597,共3页 Acta Hydrobiologica Sinica
基金 广西科技攻关项目资助(桂科攻0322006-3A)
关键词 多重聚合酶链式反应 检测 对虾 白斑综合征病毒(WSSV) 桃拉病毒(TSV) Multiplex Polymerase Chain Reaction Detection White Spot Syndrome Virus Taura Syndrome Virus
分类号 Q78 [生物学—分子生物学] S941.53 [农业科学—水产养殖]
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参考文献7

  • 1Wongteerasupaya A C,Vickers J E,Sriuairatana S, et al. A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon [J]. Dis. Aquat . Org. 1995,21:69-71.
  • 2Nunan L M, Poulos B T, Lightner D V. Reverse transcription polymerase chain reaction (RT-PCR) used for the detection of Taura Syndrome Virus (TSV) in experimentally infected shrimp [J]. Dis Aquat Org, 1998,34:87-91.
  • 3Nadala E J, Tapay L M, Cao S. Detection of yellow head virus and Chinese baculovirus in penaeid shrimp by the western blot technique[J]. J Virol Methods,1997,69:39-44.
  • 4Pang Y,Wang H, Girshick T,Xie Z, et al. Development and application of a multiplex polymerase chain reaction for avian respiratory agents[J]. Avian Dis. 2002,46(3) :691-699.
  • 5谢芝勋,庞耀珊,何竞铭,邓显文,唐小飞,刘加波,谢志勤,廖敏,文雪.应用多重PCR检测鉴别对虾白斑综合征病毒和桃拉病毒[J].中国兽医学报,2005,25(1):13-15. 被引量：11
  • 6Xie Zhixun, Avian A F,Theodore G, et al. Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction[J]. Avian Disease, 1997,41:645-660.
  • 7Sambrook J., Fritsch E. F., and Maniatis T. Molecular cloning: A Laboratory Manual [ M ], 2nd Ed. New York: Cold Spring Harbor Laboratory Press, 1989,9880-9898.

二级参考文献9

  • 1Wongteerasupaya A C,Vickers J E ,Sriuairatana S,et al. A nonoccluded,systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon[J]. Dis Aquat Org, 1995,21,69-71.
  • 2l,ightner D V, Redman R M,Poulos B T,et al, Taura syndrome ; etiology, pathology, hosts and geographic distribution,and detection methods[A], In;New approaches to viral diseases of aquatic animals[M]. Kyoto,Japan, 1997.190-205.
  • 3Office International DES Epizooties, World Organisation for Animal Health, OIE Diagnostic Manualfor Aquatic Animal Deseases[M], Third Edition,In Paris,2000.211-219.
  • 4Lightner D V ,Redman R M ,Poulos B T,et al, Risk of spread of penaeid shrimp viruses in the Americas by the international movement of live and frozen ,shrimp[J], Rev Sci Tech, 1997,16(1):146-160.
  • 5Arun K D,Michelle M R,Kurt R K. Detection and quantification of infectious hypodermal and hematopoietic necrosis virus and white spot virus in shrimp using real-time quantitative PCR and SYBR green chemistry [J]. J Clin Microbiol, 2001,39:2 835-2 845.
  • 6Nunan L M,Poulos B T,Lightner D V. Reverse transcription polymerase chain reaction(RT-PCR) used for the detection of Taura Syndrome Virus (TSV) in experimentally infected shrimp[J]. Dis Aquat Org, 1998,34: 87-91.
  • 7Nadala E J ,Tapay L M,Cao S. Detection of yellow head virus and Chinese baculovirus in penaeid shrimp by the western blot technique[J]. J Virol Methods, 1997,69 : 39-44.
  • 8Sambrook J,Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual[M]. 2nd Ed. New York:Cold Spring Harbor Laboratory Press, 1989.
  • 9Xie Zhixun,Avian A F,Theodore G,et al. Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction[J]. Avian Dis,1997,41:645-660.

共引文献10

同被引文献65

引证文献4

二级引证文献15

水生生物学报
2005年 第5期

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