摘要
目的研究牙髓卟啉单胞菌(porphyromonasendodontalis,Pe)ATCC35406对成骨细胞表达细胞核因子kappaB受体活化因子配基(receptoractivatorofnuclearfactor-kappaBligand,RANKL)的影响,探讨该菌诱导牙槽骨吸收的病理机制。方法采用原代培养的大鼠头盖骨细胞,分别以107、109CFU/ml的Pe感染细胞24h,为了观察细菌毒性作用的动力学变化,以107CFU/ml的Pe作用细胞0、6、12、18及24h,逆转录-聚合酶链式反应(RT-PCR)方法检测RANKLmRNA的表达。结果以107和109CFU/mlPe作用成骨细胞24h后,细胞表达RANKLmRNA与βactin的mRNA灰度值的比值分别为1.73、2.44。大鼠头盖骨细胞基础表达RANKLmRNA为0.32。以107CFU/mlPe感染细胞,随着感染时间的增加,细胞RANKLmRNA表达逐渐增强为0.93、2.01、1.97、1.73。结论Pe通过刺激成骨细胞RANKL的表达,激活OPG/RANKL/RANK(骨保护因子osterprotegerinOPG,细胞核因子kB受体活化因子receptoractivatorofnuclearfactor-kappaB,RANK)传导系统,能够诱导牙槽骨吸收,并且其作用具有密度依赖性和时间依赖性。
Objective：To study the effects of re（：eptor activator of nuclear factor -kB ligand -RANKL in osteoblasts （OBs） activiated by Porphyromonas endodontalis（Pe） ,and to investigate the bone resorptive pathogenesis induced by Pe. Methods： Primary rat calvarial osteoblasts were cultured, then were infected with Pe ATCC 35406 at the density of 10^7 and 10^9 CFU/ml respectively for 24 h. To determine the kinetic effect of Pe on OBs, 10^7 CFU/ml of Pe was used to stimulate the cells for 0,6,12,18 and 24 h respectively, RT-PCR were used to examine the RANKL mRNA expression. Results：The grey level ratio of RANKL mRNA expression to β-actin in untreated OBs was 0.32, that in the cells infected by 10^7 and 10^9 CFU/ml of Pe for 24 h was increased to 1.73 and 2.24 respectively. Infected by 107 CFU/nll of Pe for 6,12 and 18 h,the ratio was 0.93,2.01 and 1.97 respectively. Conclusion：Pe may stimulate RANKL expresson in osteoblasts.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2005年第5期583-586,共4页
Journal of Practical Stomatology
