摘要
目的研究过量氟对大鼠牙髓细胞表达bFGF的影响。方法选择20只Wistar大鼠,随机分为2组I组(对照组)和II组(实验组)。8周后处死动物,利用磨片、HE染色、免疫组化染色技术观察过量氟对大鼠切牙形态及bFGF表达的影响。结果实验组大鼠切牙釉质、牙本质生长线明显,牙本质可见钙质小球,牙髓细胞及髓腔内侧牙本质bFGF表达明显弱于对照组,差异有显著性(P<0.01)。结论过量氟可抑制牙髓细胞表达bFGF,影响牙体硬组织的结构。
Objective：To observe the effect of overdose fluoride on the expression of bFGF in rat＇s dental pulps. Methods：NaF at 20 mg/（ kg · d） was given to each of 10 Wister rats by stomach perfusion for 8 weeks, another 10 rats were given the same volume of distilled water as the controls. After treatment, HE staining was used to observe the morphology of the teeth, immunohistoehemical staining was adopted for study the expression of bFGF in the dental pulps of the rats. Results：The enamel layer and dentin layer of the incisors in NaF treated rats were thinner than those in the controls, the interglobular dentin was increased and the expression of bFGF in dental pulp and inner dentin was inhibited in the NaF treated rats（ P 〈0. 01 ）. Conclusion：The overdose fluoride can inhibit the secretion of bFGF and lead to the abnormal development of teeth.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2005年第5期641-644,共4页
Journal of Practical Stomatology
