摘要
目的:探讨CD44基因内含子9mRNA(CD44 IN9)与外显子V6 mRNA(CD44V6)在子宫内膜癌中的表达及在子宫内膜癌发生、发展中的意义。方法:采用RT-PCR法对10例正常子宫内膜、10例子宫内膜不典型增生、31例子宫内膜癌组织中CD44 IN9与CD44V6的表达进行检测。结果:CD44 IN9在正常子宫内膜组织中无表达,在子宫内膜不典型增生、子宫内膜癌组织中有表达,2组相对含量比较差异有显著性(P<0.05)。从正常子宫内膜到子宫内膜不典型增生及子宫内膜癌均有CD44V6表达,且其表达率和相对含量依次递增,3组间比较有极显著差异(P<0.01)。子宫内膜癌组,CD44 IN9和CD44V6表达间有一致性(Kappa值>0.4,P<0.05),CD44 IN9表达者CD44V6相对含量较CD44 IN9无表达者显著升高(P<0.05)。结论:CD44 IN9及CD44V6的异常表达可能与子宫内膜癌的发生有关。
Objective： To explore the relationship between the expressions of CD44 intron 9 mRNA （CD44IN9） and CD. exon v6 mRNA （CD44V6） and the carcinogenesis and development of endometrial carcinoma. Methods： A reverse transcription polymerase chain reaction （RT-PCR） was used to detect the expressions of CD44IN9 and CD44V6 in 3 groups： 10 cases of normal endometrium （NE） tissues, 10 cases of atypical hyperplasia endometrium （AH） tissues, and 31 cases of endometrial carcinoma （EC） tissues. Results： There was no expression of CD44IN9 in NE group. But CD44IN9 was expressed in AH and EC group, and there was significant difference in the relative content of CD44IN9 between AH and EC group （ P 〈0.05）. CD44V6 was expressed in all 3 groups. The expression rate and relative content of CD44V6 increased in the following sequence： NE, AH, and EC group, and there was significant difference among these 3 groups （ P 〈 0.01 ） . The expressions of CD44IN9 and CD44IN9 were consistent in EC group （ Kappa value 〉 0.4, P 〈 0.05）. The relative content of CD44V6 in EC group with CD44IN9 expression was higher than those without CD44IN9 expression （ P 〈 0.05）. Conclusion： The abnormal expression of CD44IN9 and CD44V6-may play an important role in the carcinogenesis and development of endometrial carcinoma.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2005年第5期475-477,共3页
Journal of China Medical University
