期刊文献+

颅脑损伤后血脑屏障形态学与通透性变化规律的实验研究 被引量:6

Experiment on the regularity in morphology and permeability of blood-brain barrier following traumatic brain injury
原文传递
导出
摘要 目的研究脑损伤后血脑屏障形态结构与通透性并评价其在血管源性脑水肿中所起的作用。方法用电镜观察血脑屏障的超微结构及镧示踪显示紧密连接的开放状态;用干湿重法测定脑组织含水量。结果伤后3~24h见内皮细胞吞饮小泡明显增多及线粒体肿胀模糊、核皱缩,基膜断裂,胶质细胞足突肿胀模糊,血管外隙扩大并液体积聚。伤后48~72h吞饮小泡增多减弱;镧颗粒示紧密连接于伤后3h初步开放,24~48h开放达高峰,72h显著开放;脑组织含水量:与对照组比较,伤后3、24h差异有显著意义(P<0.05),48h差异有非常显著意义(P<0.01),72h差异有显著意义(P<0.05)。结论伤后24h内血脑屏障通透性增高以内皮细胞吞饮活跃为主,伤后24h后以紧密连接开放为主;在血管源性脑水肿的形成过程中,紧密连接开放比吞饮活动所起作用要大。 Objective To evaluate the role of morphologic structure and permeability of BBB after traumatic brain injury in forming vasogenic brain edema. Methods The ultrastructure of BBB was observed with electron microscope and the opening state of tight junction of endotheliocyte was revealed using lanthanum trace labeling with electron microscopy. At the same time, water content of cerebral tissue was gotten by dry-wet weight means. Results On the one hand, the number of pinocytotic vesicle in endotheliocyte was greatly growing in 3h to 24h after injury,simultaneously,tumid and blurry mitochondria, wizened nucleus of endotheliocyte, rifted basilemma, tumid and blurry neurodendrite of gliocyte, extensive space outside capillary vessel and liquid accumulating could be seen in traumatic group. However,the growing in the number of pinocytotic vesicle lessen in 48h to 72h after injury. Moreover, by lanthanum trace we observed tight junction preliminary opening at 3h after trauma,at its top of opening within 24h to 48h after trauma, a large number opening still at 72h after trauma in traumatic group. On the other hand,water content of cerebral tissue in traumatic group aggravated obviously compared with control group, having obvious difference( P 〈 0.05 ) at 3 h, 24h, 72h after trauma, whenas, having very obvious differenence( P 〈 0.01 ) at 48h after trauma. Conclusions Within 24h after trauma active pinocytosis of endotheliocyre rakes more important role in inducing high permeability of BBB. Then, its chief station is replaced gradually by opening of tight junction of endotheliocyte after 24h after trauma. In a word, the opening of tight junction of endotheliocyte maybe take greater effect than active pinocytosis of endotheliocyte in forming vasogenic brain edema.
出处 《中国基层医药》 CAS 2005年第9期1124-1126,共3页 Chinese Journal of Primary Medicine and Pharmacy
基金 广东省科技计划资助项目(2002C31002)
  • 相关文献

参考文献8

二级参考文献12

  • 1王振宇 李学荣 等.用硝酸镧示踪法观察渗透性血脑屏障开放后的超微结构变化[J].第四军医大学学报,1986,7(4):277-280.
  • 2Yang G Y,Stroke,1994年,25卷,1658页
  • 3Xue D,Brain Res,1992年,587卷,66页
  • 4王振宇,第四军医大学学报,1986年,7卷,4期,277页
  • 5D. F. Condorelli,V. G. Nicoletti,P. Dell’Albani,V. Barresi,A. Caruso,S. G. Conticello,N. Belluardo,A. M. Giuffrida Stella. GFAPβ mRNA Expression in the Normal Rat Brain and after Neuronal Injury[J] 1999,Neurochemical Research(5):709~714
  • 6H. H. Kaufman,J. L. Pruessner,D. P. Bernstein,A. Borit,P. T. Ostrow,D. L. Cahall. A rabbit model of intracerebral hematoma[J] 1985,Acta Neuropathologica(3-4):318~321
  • 7曹晓哲,高自芳,罗晓阳,邢传平,钱震.实验性脑损伤后肺硝酸镧示踪的电镜观察[J].中华物理医学杂志,1997,19(1):41-42. 被引量:2
  • 8杨树源,杨学军.重型颅脑损伤的监护[J].中华创伤杂志,1999,15(3):167-168. 被引量:23
  • 9刘兴波,衣服新,罗俊生,王秋华.二次脑创伤临床诊断指标的初步探讨[J].锦州医学院学报,2002,23(3):1-3. 被引量:5
  • 10李学荣,王振宇.镧醛灌注固定血脑屏障通透性的电镜技术[J].第四军医大学学报,1992,13(5):386-387. 被引量:6

共引文献49

同被引文献62

引证文献6

二级引证文献27

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部