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桃幼胚及幼胚子叶再生的研究 被引量:12

Plantlets Regeneration from Immature Embryos and Cotyledons of Peach
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摘要 以玉露桃和湖景蜜露桃幼胚及幼胚子叶为试材,研究基本培养基类型、激素、损伤方式等因素对再生的影响。结果表明:培养基是影响幼胚诱导愈伤组织的最重要因素,MS适合供试材料诱导愈伤组织;激素诱导愈伤组织因品种而异,NAA1.0mg·L-1对45d、0.5mg·L-1对55d玉露,BA0.5mg·L-1对55d湖景蜜露效果较好,在试验浓度范围内2,4-D对供试幼胚均无明显效果;幼胚的发育状态是影响诱导愈伤组织的另一因素,玉露桃45d愈伤组织诱导率最高,达96.6%,而55d诱导率为81.6%。愈伤组织可在MS+0.05mg·L-1NAA+1.0mg·L-1BA培养基上分化成芽,再生芽在1/2MS+1.0mg·L-1IBA培养基上生根。幼胚子叶不定芽再生培养基的激素配比为NAA0.50mg·L-1+BA10.0mg·L-1、NAA0.05mg·L-1+TDZ3.0mg·L-1;带胚芽子叶纵向刻伤再生率高;不定梢(芽)在1/2MS+2%蔗糖+7.5g·L-1琼脂+IBA1.0mg·L-1+Ad20mg·L-1的培养基上能生根。 The main composition of culture media such as the basic medium and hormones were tested for their effects on Peach (Prunus persica ) callus frequency from immature embryos (which picked after full flower 4.5 days and 55 days respectively) under (26± 1) ℃ without light in an orthogonal experiment. And a method for plant regeneration from immature cotyledons (55 d) has been developed by cultured on MS media supplemented with 2% sucrose and 0.75% agar and hormone in various combinations. The results showed that: The basal medium was the most important factor for inducing callus from embryos. MS was adapted to ‘Yulu' (45 d and 55 d) and ‘Hujingmilu' (55 d) . NAA 1.0 mg·L^-1 and 0.5 mg·L^-1 were better for ‘Yulu' (4.5 d and 55 d, respectively), BA 0.5 mg·L^- 1 was better for ‘Hujingmilu' (55 d), but 2,4-D hadn't any important effect on callus inducing from the examined material. Days after fecundation was another factor effect on callus induction. The highest callus frequency from 4.5 d immature embryos was 96.6% and it was 81.6% from 55 d embryos. Shoots regeneration occurred when callus was transferred to a medium in which the NAA concentration was 0.0.5 mg·L^-1 and BA was 1.0 mg·L^-1 . Shoot regenerated roots in the medium of 1/2 MS + 1.0 mg·L^-1 IBA. 2 types of hormone combinations (NAA 0.50 mg·L^-1 + BA 10.0 mg·L^-1 , NAA 0.0.5 mg·L^-1 + TDZ 3.0 mg·L^-1 ) were favorable to shoots differentiation for the immature cotyledons. The regeneration was changed when cotyledons were wounded horizontally by a razor blade. The best results were obtained when cotyledons with embryonic axis and wounded in the surface. Adventitious shoot could regenerate on the 1/2MS medium with IBA 1.0 mg·L^-1 andAd20 mg·L^-l.
出处 《林业科学》 EI CAS CSCD 北大核心 2005年第5期45-50,i0002,共7页 Scientia Silvae Sinicae
基金 宁波市科委项目"桃转基因研究"(2000农业项目资助) 浙江省农业科学院人才引进项目资助。
关键词 幼胚 子叶 愈伤组织 离体再生 Peach( Prunus persica ) immature embryos cotyledons callus regeneration
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参考文献10

  • 1吴延军,徐昌杰,张上隆.桃组织培养和遗传转化研究现状及展望[J].果树学报,2002,19(2):123-127. 被引量:38
  • 2吴延军,张上隆,张岚岚,沈淦卿,吴大军.桃幼胚子叶不定芽发生的初步研究[J].浙江大学学报(农业与生命科学版),2003,29(1):93-96. 被引量:8
  • 3阎国华,周宇.桃幼胚下胚轴高频植株离体再生[J].果树学报,2002,19(4):231-234. 被引量:21
  • 4张永庆,陈大明,金勇丰,张上隆.桃离体组织分化再生植株的研究[J].园艺学报,2001,28(4):342-344. 被引量:44
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  • 7Mante S, Scoria R,Cordts J M. 1989. Plant regeneration from cotyledons of Prunus persica, Prunus clomestica and Prunus cerasus. Plant cell Tissue and Organ Culture, 19(1): 1-11.
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二级参考文献19

  • 1张素,周建涛,陆维忠.桃无性系砧木GF43微繁技术研究[J].江苏农业学报,1993,9(3):51-52. 被引量:5
  • 2范子南,肖华山,范晓红,黄飞.冬桃的离体繁殖[J].植物生理学通讯,1996,32(3):199-200. 被引量:9
  • 3孟新法 周维燕.桃胚乳离体培养再生植株的研究[J].北京农业大学学报,1981,7(4):95-98.
  • 4[1]Hammerschlag F A, Bauchan G,Scourza R. Regeneration of Peach Plant from Callus Derived from Immature Embryos[J]. Theoretical and Applied Genetics, 1985, 70(3): 248-251.
  • 5[2]Scorza J M, Cordts S M. Long-term somatic embryo production and production and plant regeneration from embryo-drived peach callus[J]. Acta Horticulturae, 1990, 280: 183-284.
  • 6[3]Rizzo M, Bassi D, Byrne D, et al. Growth of Immature Peach (Prunus persica L. Batch) embryos on different media[J]. Acta Horticulturae, 1998, 465,141-144.
  • 7[4]Scorza J M, Cordts S M. Agrobacterium-mediated transformation of peach, Prunus persica L., leaf segment, immature embryos and long-term embryogenic callus[J]. IN Vitro Cellular and Developmental Biology, 1991, 26(8): 829-834.
  • 8[5]Mante S,Scorza R,Cordts J M. Plant Regeneration from Cotyledons of Prunus Persica,Prunus Domestica and Prunus Cerasus[J]. Plant Cell Tissue and Organ Culture, 1989,19:1,1-11.
  • 9[6]Pooler M R,Scorza R. Shoots Were Regeneration from Cotyledons of Mature Stored Seed of Three Peach Rootstock Cultivars Flordaguard. Nemared and Medaguard[J]. HortScience,1995,30:2,355-356.
  • 10[7]SUN Qing-rong, Sun Hong-yan, SHI Yin-ping(孙清荣,孙洪雁,石荫坪). Plant regeneration from cotyledons of mature seed of two peach cultivars[J]. Shandong Agricultural Sciences(山东农业科学), 2000, 2: 11-12. (in Chinese)

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