摘要
采用斑马鱼胚胎发育技术,对环境激素类物质五氯酚的毒性进行测定,结果表明,五氯酚(PCP)对胚胎的特定作用时间段是卵产出至发育6h之内;PCP对胚胎发育有明显的抑制作用,会造成胚胎发育的畸形或死亡,不同时间染毒产生的可观察毒理学终点各异;随着PCP对发育48h斑马鱼胚胎作用时间的减短,其致死效应敏感性降低,其中0hpf组的LC50值最小,为70.8μg·L-1,24hpf组LC50值最大,为831.8μg·L-1;斑马鱼胚胎对孵化后0时染毒的PCP最为敏感,PCP对胚胎产生急性毒性效应的敏感指标:心胞囊肿、血液循环障碍、无心律>孵化率降低>停滞发育作用;斑马鱼胚胎最敏感的指标为48h血液循环障碍和48h半致死效应.
With embryo development technique, this paper determined the toxicity of pentachlorophenol （PCP） to Brachydanio rerio embryos. The results showed that the special effect duration of PeP on embryos was within 6 hours after embryos incubation. PeP could markedly inhibit the development of Brachydanio rerio embryos, and cause its malformation and even death. There were different toxicity endpoints which could be observed in embryos exposed to PCP with different time. The lethal effect sensitivity of embryos incubated after 48 hours was getting lower when the exposure time was getting shorter, the LCs0 being the minimum （70.8 μg· L^- 1 ） for 0 hpf embryos, and the maximum （831.8 μg·L^- 1 ） for 24 hpf embryos. The acute toxicological endpoim of Brachydanio rerio embryos was in order of edema, no blood-circulation and heartbeat 〉 incubation rate〉 stopping growth, and the most sensitive endpoims of Brachydanio rerio embryos to pentachlorophenol were no blood-circulation and half lethal concentration at 48 hrs.
出处
《应用生态学报》
CAS
CSCD
北大核心
2005年第10期1967-1971,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金资助项目(30470319).
关键词
五氯酚
斑马鱼胚胎
毒性效应
Pentachlorophenol, Brachvdanio rerio, Toxicity effect.
