摘要
目的:观察补充谷氨酰胺对力竭运动大鼠血、脑单胺含量的影响及时间依赖性。方法:实验于2003-05/07在南京体育学院完成。选择8周龄雄性SD大鼠30只,随机分为3组即对照组、一次性补充谷氨酰胺组、长期补充谷氨酰胺组。每组10只。①谷氨酰胺补充:一次性补充谷氨酰胺组大鼠在运动前2h灌服淀粉谷氨酰胺糊,谷氨酰胺剂量为0.04mg/(g·d)。长期补充谷氨酰胺组谷氨酰胺的补充分为两阶段,第1阶段:灌服淀粉谷氨酰胺糊,剂量为0.04mg/(g·d),服用7天;第2阶段:灌服淀粉谷氨酰胺糊,剂量0.02mg/(g·d),服用13d。对照组大鼠每天灌服等体积量1%的淀粉糊0.02mg/(g·d)。②运动试验:大鼠在PT-98型动物跑台上运动,速度为28m/min,以不能坚持负荷跑速,滞跑道的后1/3处达3次以上,刺激驱赶无效(10s)为力竭标准。③取样及测试:大鼠运动后即刻直接断头处死,取脑组织并去除血膜、嗅球和小脑,称重;取血,分离血清,放置-80℃冰箱保存,待测。采用荧光比色法测定多巴胺、去甲肾上腺素、5-羟色胺和5-羟吲哚乙酸的含量。结果:各组大鼠均完成实验,全部进入结果分析。①力竭性跑台运动后即刻,一次性和长期补充谷氨酰胺大鼠脑组织多巴胺、去甲肾上腺素均显著性高于对照组,脑5-羟色胺和血清5-羟色胺均显著性低于对照组;一次性补充谷氨酰胺组大鼠脑5-羟吲哚乙酸显著性高于对照组,血清多巴胺显著性低于对照组。长期补充谷氨酰胺大鼠脑多巴胺和血清多巴胺显著性高于一次性补充组,而脑5-羟吲哚乙酸则显著性低于一次性补充谷氨酰胺组。②一次性补充谷氨酰胺组和长期补充谷氨酰胺组大鼠脑组织5-羟色胺/多巴胺比值显著性低于对照组;长期补充谷氨酰胺组大鼠脑组织5-羟色胺/多巴胺比值显著性低于一次性补充谷氨酰胺组。③各组大鼠血清去甲肾上腺素和血清5-羟吲哚乙酸差异没有显著性。补充谷氨酰胺显示血清中单胺类物质与脑中单胺总体上呈相同的变化趋势。结论:补充谷氨酰胺有利于提高大鼠力竭性跑台运动后脑组织多巴胺和去甲肾上腺素水平,且可降低脑内的5-羟色胺含量和5-羟色胺/多巴胺比值;长期补充谷氨酰胺可能更有利于提高运动中脑的机能;一次性补充和长期补充对脑5-羟色胺的影响可能存在不同的机制。
AIM： To observe the effects of glutamine supplement on the monoamines in serum and brain after exhaustion movement in rats and the time dependency. METHODS： The experiment was done in Nanjing Institute of Physical Education between May 2003 and July 2003. Thirty Spragne-Dawley male rats aged 8 weeks old were selected and divided into three groups randomly： control group, once glutamine supplement group and long-term glutamine supplement group with 10 rats in each group, ① Glutamine supplement： The rats in the once glutamine supplement group were supplied with starch and glutamine aleurone by perfusion, including 0.04 mg/g glutamine per day at 2 hours before the movement. Those in the long-term glutamine supplement group were supplied with glutamine in two phases： in the first phase the rats were treated with starch and glutamine aleurone by perfusion, at the dosage of 0.04 mg/g per day for 7 days; in the second phase they were treated with starch and glutamine by perfusion, at the do,sage of 0,02 mg/g per day for 13 days. Rats in the control group were supplied with 1% amylure 0.02 mg/g per day. ② Movement test： All the rats were suffered with treadmill running with speed 28 m per minute on PT-98 animal Treadmill. The exhaustion standard was the rat could not keep the speed and stagnant stay in the 1/3 back part of the treadmill even with electricity stimulation for 10 s more than 3 times. ③ Sample and test： The rats were sacrificed with decollation instantly after exhaustion. Brain tissue was sampled without blood film, olfactory bulb and cerebellum, weighted, gained the blood, and separated the serum. All samples were kept＇in -80℃ in the refrigerator till test. The concentration of dopamine （DA）, norepinephrine （NE）, 5-hydrox- ytryptamine （5-HT） and 5-hydroxyindole acetic acid （5-HIAA） were detected with fluorescence colorimetry. RESULTS： All the rats were past through the experiments and were involved in the result analysis. ① The content of DA, NE in rats brain of the once glutamine supplement group and the long-term glutamine supplement group were significantly higher than those in the control group, while the 5- HT in brain and serum were significantly lower than that in the control group. The 5-HIAA in rat brain of the once glutamine supplement group was significantly higher than that in the control group, while DA in serum were significantly lower than that in the control ,group. The DA in brain and serum of the long-term glutamine supplement group were significantly higher than that in the once glutamine supplement group, while 5-HIAA in brain were significantly lower than that in the once glutamine supplement group. ② The ratio of 5-HT/DA in brain of the once glutamine supplement group and the long-term glutamine supplement group were significantly lower than that in the control group; The ratio of 5-HT/DA of brain in the longterm glutamine supplement group was even lower than that in the once glutamine supplement group significantly. ③ The NE and 5-HIAA in serum of each groups had no significant difference. Monoamines in serum and brain seemed have the same tendency of changes with glutamine supplement. CONCLUSION： Glutamine supplement may enhance the levels of DAand NE,reduce the 5-HT content and 5-HT/DA ratio in rat brain after exhausted treadmill running; The long-term glutamine supplement may have advantageous effects on the function of brain. The once glutamine supplement and long-term glutamine supplement may have different mechanism in the effects on the 5-HT in brain.
出处
《中国临床康复》
CSCD
北大核心
2005年第36期97-99,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家体育总局应用基础研究项目(01067)~~
