摘要
新生猪胰腺经胶原酶消化，分离纯化得纯净胰岛。用ＢａＣｌ＿２交联一步法微囊技术包裹该胰岛，所得微囊直径５００～７００μｍ，每个微囊含１～３个胰岛。移植治疗Ⅰ型糖尿病５例，每例移植胰岛数为１３±２万，术后不使用免疫抑制剂。受体血糖移植前为１８．２±３．７４ｍｍｏｌ／Ｌ，用药后降至８．１４±２．０６ｍｍｏｌ／Ｌ，血清Ｃ肽由１１３．５±５８ｐｍｏｌ／Ｌ升至２４７±８３ｐｍｏｌ／Ｌ。外源胰岛素用量显著减少，从移植前的６４±１５．４８Ｕ／ｄ减至２７．２±９．４４Ｕ／ｄ。提示采用微囊化新生猪胰岛异种移植，能有效地纠正患者高血糖，显著减少胰岛素用量。证明海藻酸钠微囊具有良好的免疫隔离作用和生物膜性能，可有效地避免免疫排斥反应。
The pancreata of new born pigs were digested by collagenase and then isolated to collect pure islets,these islets were prepared to microcapsules by Bariumcross linked one-step method with alginate. The diameter of microcapsules is 500-700μm, and every capsule cantains 1-3 pancreatic islets. The capsules were transplanted to five IDDM patients without immunodepressive. After transplantation fasting blood sugar of the patients decreased from 18.2± 3.74mmol/L to 8.14 ±2.06mmol/L. The dosage of injection insulin reduced from 64±15- 48U/d to 27.2 ± 9.44U/d, serum C peptide increased from 113.5± 58pmol/L to 247±83pmol/L. The curative effect was maintained for more than a hundred days.The results reveals that the graft of microencapsulated pancreatic islets effectively correct the blood sugar of IDDM. It also shows that themicroencapsulation had the immunoisolation and biomembrane function, it can protect the host from antigraft rejection.
出处
《山东医科大学学报》
1996年第2期112-116,共5页
Acta Academiae Medicinae Shandong
关键词
胰岛
糖尿病
胰岛素依赖性
微囊
移植
新生猪
Islands of langerhans
Diabetes millitus, insulin-dependent:Microencapsulation
Transplantation, heterogenous
New born pigs
Human
