摘要
通过单因素实验和正交实验,对铜绿假单胞菌发酵生产鼠李糖脂的培养基组成及培养条件进行了研究,结果表明:优化后的培养基组成为(g/L):NaNO38,KH2PO40.8,K2HPO41.0,MgSO42.5,MnSO40.015,FeSO40.02,酵母膏1.5,橄榄油14%。最佳培养条件为:250ml三角瓶的装液量60ml,接种量10%,摇床培养温度35℃,转速150r/min,初始pH7.5,种子培养1d,发酵培养4d。在此条件下,发酵液的最大产鼠李糖产量为21.06g/L,比优化前提高了31.7%。
The effects of fermentation conditions on rhamnolipid produced by Pseudomonas aeruginosa were investigated through the single factor and orthogonal experiments. The results showed that： The optimized compositions of the medium were （g/L）： NaNO38, KH2PO40.8, K2HPO41.0, MgSO4 2.5, MnSO4 0.015, FeSO4 0.02, yeast extract1.5, and olive oil14%. The optimum conditions were： 60ml in 250ml total volume, seed volume 10%（VN）; the temperature of the shaking table 35℃, rotating speed 150r/min, the initial pH7.5, the culture time of seed 1d, and the fermentation time 4d. Under the optimum conditions, the maximum yield of rhamnose was 21.06g/L which it was increased by 31.7%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第10期62-66,共5页
Food Science
基金
河南省教育厅自然科学研究项目(20011800006)
关键词
鼠李糖脂
鼠李糖
发酵
优化
rhamnolipids
rhamnose
fermentation
Optimize
