摘要
对筛选到的一组纤维素分解菌复合系WSC-6,通过变性梯度胶电泳(DGGE)方法研究了菌种的组成稳定性.结果表明,在连续继代培养的第74～83代复合系的菌种组成没有变化,非常稳定.多代继代培养过程中各代的pH值变化趋势一致,pH值从发酵开始的8.7下降到纤维素旺盛分解时的6.5以下;随着分解结束,pH值逐渐恢复到发酵开始时的水平并保持稳定,具有较强的自我调节能力.多代继代培养后复合系各代的滤纸纤维素分解率和CMC糖化差异很小;在发酵液起始pH 4～10的范围内,复合系对pH值具有缓冲能力,并正常分解纤维素;经过70～100℃高温处理10min后再转接的复合系对纤维素仍然具有分解能力,功能稳定.
For a group of cellulose degradation bacteria composite system WSC-6 obtained through screening, the stability of composition of bacteria strain was studied through technique of denaturing gradient gel electrophoresis （DGGE） technique. The composition of bacteria strain of 74-83 generations of continuous subculture in composite system did not change, with special stability of the composition. The pH value change of each generation in the process of subcultures tended to be same. Value of pH decreased from 8.7 of the beginning of enzyme formation to lower than 6.5, when the cellulose was degraded actively, and then resumed gradually to the initial level towards the end of degradation and maintained stability, and possessed self-regulation ability. The degradation rate of the filler paper cellulose and CMC scarification in each generation of composite system after sub-cultures differed very little. In pH 4-10 range at the beginning of enzyme forming liquor composite system possessed buffer ability and degraded cellulose normally. After treatment of high temperature 70℃ to 100℃ for 10min, the composite system of turn-contact strain possessed still the degradation ability of cellulose, with very stable function.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2005年第5期567-571,共5页
China Environmental Science
基金
国家"863"资助项目(2002AA245031)
国家"十五"科技攻关资助项目(2002BA516A03)
关键词
纤维素分解
微生物复合系
稳定性
变性梯度胶电泳
cellulose degradation
composite microbial system
stability
denaturing gradient gel electrophoresis （DGGE）
