摘要
目的:建立HPD系列大孔吸附树脂的预处理方法.方法:采用气相色谱法(GC)和紫外分光光度法比较了2%NaOH对HPD系列大孔吸附树脂预处理的影响.结果:树脂先用2%NaOH浸泡后再用乙醇洗脱,对有机残留物的洗脱效果较好.结论:HPD系列树脂预处理方法为:树脂经2%NaOH浸泡洗脱后,在60℃,用乙醇浸泡、洗脱,洗脱流速为2 BV·h-1,乙醇用量为3～4倍柱体积,洗脱液的紫外吸收值为0.2～0.5.预处理的树脂经GC检测,苯未检出,其他有机残留物均低于10 mg·L-1,达到药用要求.
Objective： To develop a method for the pretreatment of the series of HPD macroporous adsorption resin（MAR）. Method： The effect of 2% NaOH to pretreatment of the series of HPD MAR was studied by gas chromatography and UV spectrometery. Result： The eluting effect by ethanol was better after the MAR was marinated and eluted by 2% NaOH. Condutlon： The pretreatment of the series of HPD MAR was：after marinated and eluted by 2%NaOH, the series of HPD MAR were eluted by ethanol at 2 BV·h^-1 at 60℃ for 3-4 bed volumes （BV）. At then the UV absorbance of eluate was 0.2-0.5. Benzene was not detected and the other residues less than 10 mg·L^-1 in elutedMAR by GC. And the MARs match to the standard of medicine.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第18期1425-1427,1470,共4页
China Journal of Chinese Materia Medica
基金
国家"十五"科技攻关项目(2001BA701A02)
关键词
气相色谱法
HPD系列大孔吸附树脂
预处理
甲苯
萘
gas chromatography
series of HPD macroporous adsorption resin
pretreatment
toluene
naphthalene
