摘要
建立反相高效液相色谱(HPLC)法测定杜仲叶发酵液中绿原酸的含量。色谱条柱SymmetrySheildRP18柱(3.9mm×300mm,5μm),以水-甲醇-乙酸(88∶12∶1.2,V/V)为流动相,流速1.0ml/min,检测波长326nm,柱温25℃。绿原酸对照品在2.24～11.2μg范围内线性关系良好(r=0.9993)。该方法简便、准确、线性范围宽,可用于含绿原酸的原料及产品的质量控制。
A determination method of chlorogenic acid by high performance liquid chromtograph and photodiode array detector was established. The chromatographic column, Symmetry Sheild RP 18 （ 3.9 mm× 300 mm, 5μm ）, water -methanol-acetic acid （ 88：12：1.2, V/V ） mobile phase with 1.0 ml/min flow rate, the detected wavelength （326 nm ）, and the column temperature（ 25℃） were adopted. The calibration curve of chlorogenic acid was linear under the content of 2.24～11.2μg, the correlation coefficient was 0.999 3. The method was simple, accurate and had wider linear range. The method can be adapted to control the quality of material and products that contained chlorogenic acid.
出处
《安徽农业科学》
CAS
北大核心
2005年第10期1862-1863,共2页
Journal of Anhui Agricultural Sciences
基金
江西省教育厅重点资助项目。
关键词
反相高效液相色谱法
杜仲叶
发酵液
绿原酸
RP-HPLC
Eucommia ulmoides Oliv. leaf
Fermented liquor
Chlorogenic acid
