摘要
以发育的玉米胚为材料,用酶联免疫吸附检测技术测定了玉米胚发育和脱水过程中内源ABA和GA3含量的动态变化,以及它们与胚的脱水耐性形成的关系.结果表明,玉米胚在发育过程中其ABA含量峰值出现在GA3含量峰值之前,而且ABA峰值出现在授粉后28 d,晚于胚的含水量下降;胚在脱水过程中ABA和GA3的含量都呈先上升后下降的变化.可以认为在脱水过程中ABA含量的增加是胚对脱水胁迫应激反应的结果,有保护胚的作用;但GA3含量增加的生理机制及其作用尚不清楚.在玉米胚的发育过程中,内源ABA和GA3之间的相对平衡可能主要调控胚的发育进程和萌发力的形成;胚的ABA含量变化可能不足以引起其脱水耐性的形成.
Enzyme linked immunosorbent assay（ELISA） was adopted to determine the contents of endogenous ABA and GA3 of maize embryos in their development and dehydration for studying their relationship with dehydration tolerance formation of the embryos. The results illustrated that in the development of maize embryos,the ABA content of the embryos came to its highest point before the GA3 content did and that its peak appeared on the twenty eighth days after pollination ,which was later than the time when the water content of the embryos declined;the ABA and GA3 contents of the embryos appeared to initially increase and then decline in their dehydration. Accordingly,the ABA increase of the embryos in their dehydration was a response to the stress of the dehydration and thus helpful to protect the embryos;but the biological mechanism and effects of the GA3 increase were still not clear. In the development of maize embryos, the balance between endogenous ABA and GA3 in the embryos may mainly regulate the developmental progress and germinating force formation.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第9期1772-1777,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
中国科学院知识创新工程重要方向性项目(KSCX2-SW-117)
中国科学院"百人计划"项目
科技部国家科技基础条件平台项目(2004DKA30430)
