摘要
目的: 探讨肾移植术后高脂血症患者外周血RANTES (regulated on activation normal T-cell expressed and secreted)及其受体CCR5(chemokine receptor 5) mRNA表达及治疗. 方法: 根据患者肾移植术后6～8 mo的血脂水平分血脂正常组(n=30)和血脂增高组(n=30),血脂增高组又根据辛伐他汀治疗时间分1.5 mo治疗组(n=30)和3 mo治疗组(n=30),并设正常对照组(n=30),利用RT-PCR检测各组患者外周血RANTES和CCR5 mRNA表达水平. 结果: 各组肾功能正常,胆固醇酯,三酰甘油,低密度脂蛋白水平均按对照组,血脂正常组,血脂增高组顺序递增(P<0.05),3 mo治疗组较1.5 mo治疗组有显著降低(P<0.05);RANTES及其受体CCR5 mRNA表达水平按对照组,血脂正常组,血脂增高组顺序递增,1.5 mo治疗组和3 mo治疗组均较血脂增高组有显著降低(P<0.05),而3 mo治疗组又明显低于1.5 mo治疗组(P<0.05). 结论: RANTES及其受体CCR5 mRNA表达可能在移植肾慢性损伤早期起作用;高脂血症能加重已增高表达,辛伐他汀治疗可降低表达.
AIM： To study whether hyperlipidemia after renal transplantation is related to the expression of RANTES （ regulated on activation normal T-cell expressed and secreted） and its receptor CCR5 （chemokine receptor 5） mRNA in peripheral monocyte cells （PMCs） and to explore its therapy. METHODS： Renal recipients were divided into normal lipidemia group （ n = 30） and hyperlipidemia group （n=30） according to the serum lipid level 6 -8 months after transplantation. The patients in the hyperlipidemia group were treated with simvastatin for 1.5 months （ 1.5-month treatment group） or 3 months （3-month treatment group）. The control group consisted of 30 healthy subjects. Serum total cholesterol （TC）, triglyceride （ TG ）, low-density lipoprotein （LDL）, high-density lipoprotein （HDL） were measured and RT-PCR was used to detect the expression of RANTES and CCR5 mRNA in PMCs. RESULTS： The renal function of all the patients was normal. TC, TG, LDL and expression of RANTES and CCR5 mRNA in the normal lipidemia group were lower than those in the hyperlipidemia group, but higher than those in control group （ P 〈 0.05 ）, and those in 3-month treatment group were significantly lower than those in 1.5-month treatment group （ P 〈 0.05 ）. CONCLUSION： The expression of RANTES and CCR5 mRNA is involved in the early-stage chronic injury of kidney allografts. Hyperlipidemia can strengthen the increased ex- pression of RANTES and CCR5 mRNA. Simvastatin can reduce the expression of RANTES and CCR5 mRNA in the kidney transplant recipients with hyperlipidemia.
出处
《第四军医大学学报》
北大核心
2005年第19期1787-1789,共3页
Journal of the Fourth Military Medical University
