摘要
目的观察葡多酚(GPC)对正常肝细胞及受乙醇损伤的肝细胞增殖活性的影响。方法将小鼠正常肝细胞和乙醇引发损伤的肝细胞加入GPC培养,用四甲基偶氮噻唑蓝(MTT)法测定各组肝细胞增殖活性。将GPC经口给予正常小鼠和乙醇引发急性肝损伤的小鼠,取肝脏匀浆后培养3和16 h,用前述方法测定肝细胞增殖活性。结果体外实验的正常对照组和乙醇对照组细胞增殖活性分别为0.438±0.040和0.365±0.024;50 mg/L GPC保护组和50 mg/L GPC对照组则为0.541±0.026和0.603±0.060。体内实验的150 mg/L GPC保护组细胞增殖率较乙醇对照组升高0.095;150 mg/L GPC对照组则较正常对照组升高0.030。各组之间的差异均有统计学意义(P<0.05)。结论GPC能抑制乙醇对肝细胞增殖活性的损伤,并对正常肝细胞增殖活性有一定促进作用。
Objective To study the effect of GPC on the proliferation of normal hepatoeytes and the cells damaged by ethanol. Methods ①Normal primary mouse hepatocytes and the cells damaged by ethanol were cuhrued with grape procyanidins（GPC）of different concentrations. Methyl thiazolyl tetrazolium（MTT） assay was used to analyze the cell proliferation. ②GPC was given to normal mouse and the ones with liver injuried by ethanol acutively through mouth. Cell proliferations of 3 h and 16 h were analyzed by the same way as above. Results In vitro the proliferations of normal control and the ethanol group were 0.438±0.040 and 0.365±0.024 respectively. Those of the 50 mg/L GPC protective group and control group were 0.541±0.026 and 0.603±0.060 respectively. In vivo, the proliferation rates of the 150 mg/L GPC protective group were 0.095 higher than that of the ethanol one. That of the 150 mg/L GPC control group was 0.030 higher than that of the normal control one. All differences above were significand （ P 〈 0.05 ）. Conclusion GPC can inhibit the damage of ethanol on proliferation of hepatocytes, and promote proliferation of normal cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第11期1307-1308,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30271127)
