摘要
用花烛华伦天努的幼嫩叶片诱导产生愈伤组织,以1/2 MS+6-BA 1.0 mg/L+2,4-D 0.5 mg/L的幼叶出愈伤率最高,达到92%;愈伤组织芽的分化,以1/2 MS+6-BA 1.5 mg/L+IAA 0.3 mg/L的组合培养基最适宜于愈伤组织芽的分化,诱导率达到94%以上;在相同激素水平条件下,1/2 MS对不定芽转化为正常苗有利,NAA 0.1～1.0 mg/L促进生根,所获试管苗移栽成活率达93%,商品出苗率达90%.
Young leaves of Anthurium andraeanum variety Valentino were used as explants in in vitro culture. The optimum medium for callus induction was 1/2 MS ＋ 6 - BA 1.0 mg/L ＋ 2,4 - D 0.5 mg/L, which gave an induction rate of 92%. The medium 1/2 MS ＋ 6 - BA 1.5 mg/L ＋ IAA 0.3 mg/L gave the highest rate of shoot differentiation （≥94%）. The addition of NAA at 0.1 ～ 1.0 mg/L promoted the rooting of the induced shoots. With similar hormone concentration in the medium, the reduction of the content of macro - elements was helpful for shoot differentiation and growth. Over 93% of the tube plantlets survived after transplantation.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2005年第5期612-615,共4页
Journal of Southwest Agricultural University
关键词
花烛
离体培养
植株再生
Anthurium andraeanum
in vitro culture
plantlet regeneration
