摘要
以水稻品种中花8号的悬浮细胞为试材,分别研究了真菌激发子和盐激发子对用稻瘟灵(IPT)和脱落酸(ABA)预处理24 h的水稻悬浮细胞中PBZ1基因表达的影响.结果发现:单独用IPT、ABA处理可以诱导细胞中PBZ1基因表达;只用真菌激发子和盐激发子处理的细胞中也可以检测到PBZ1基因的表达,但两种激发子对PBZ1基因表达的影响不同,在真菌激发子处理后的0～3 h之间PBZ1基因表达逐渐增强,而在盐激发子处理后的0～3 h之间则呈现逐渐减弱的趋势,到第3 h就检测不到了;用IPT预处理24 h后,再分别用真菌激发子和盐激发子诱导,水稻细胞中PBZ1基因表达强度都显著高于单独用IPT处理的细胞,而且在处理后的第0.5～3 h期间一直保持较强的表达;ABA+盐激发子处理使细胞中PBZ1基因表达推迟且短暂,只在处理后第1～2 h之间可检测到,ABA+真菌激发子处理使细胞中PBZ1基因在0.5～3 h期间一直保持较强的表达,明显强于单独用ABA处理的细胞.可见,IPT和ABA都可以诱导抗病基因PBZ1的表达,PBZ1基因也可由盐激发子诱导表达,说明PBZ1基因对除病害以外的非生物逆境也有积极的作用.
The effect on expression of PBZ1 in rice suspension cell （ Zhonghua 8 ） induced by the elicitors of fungal and salt pretreated with isoprothiolane （IPT） and abscisic acid （ ABA ） for 24 h were studied. The results showed that IPT or ABA or the two elicitors treatment could separately induce the expression of PBZ1 in the suspension cell and without the expression of PBZ1 in the control cells was observed treated with only the fungal and salt elicitors. The expression of PBZ1 enhanced gradually from zero to three hours after treated with fungal elicitor and weakened gradually to zero within the same interval treated by salt elicitor. The expression intensity of PBZ1 in rice cell treated only with IPT was obviously higher than that of induced by elicitor of fungal or salt pretreated by IPT for 24 h, and a continuance of high expression of PBZ1 was observed from 0.5 to 3h after treatment as well as by fungal elicitor after ABA treatment. It is obvious that IPT, ABA and fungal could induce the expression of PBZ1. The PBZ1 gene had active effects on the abiotic stresses with the exception of diseases.
出处
《农药学学报》
CAS
CSCD
2005年第3期254-258,共5页
Chinese Journal of Pesticide Science
关键词
抗逆诱导剂
稻瘟灵
脱落酸
PBZ1基因
stress-resistance inducer
isoprothiolane （IPT）
abscisic acid （ABA）
PBZ1
