摘要
目的:探讨淋巴瘤骨髓受累的免疫表型特征。方法:采用流式细胞仪CD45/SSC设门方法对34例恶性淋巴瘤患者的骨髓标本进行检测,以骨髓涂片细胞学检查作阳性对照。收集骨髓受累患者的CD分子表达数据。结果:①对34例恶性淋巴瘤患者的骨髓应用流式细胞仪进行检测,发现23例阳性,阳性率67.65%(23/34),95%可信区间(51.92%,83.37%)。②该23例阳性患者中,非霍奇金淋巴瘤(NHL)19例,霍奇金淋巴瘤(HL)4例。NHL患者中B细胞来源免疫荧光单克隆抗体标记抗原出现频率最高的为CD19,CD20;T细胞来源标记抗原出现频率最高的为CD7。而在HL患者中出现频率最高的为CD9。结论:采用流式细胞仪CD45/SSC设门方法,发现非霍奇金淋巴瘤骨髓受累患者免疫表型特征为:B细胞来源:CD19、CD20;T细胞来源:CD7。霍奇金淋巴瘤为:CD9。
Purpose：To analyze the immunophenotype character of malignant lymphoma with bone marrow involvement by means of Flow Cytometry（CD45/SSC gate）.Methods：Bone marrow of malignant lymphoma patients was detected by Flow Cytometer（CD/SSC gate）,bone marrow smear was simultaneously performed as control.Resuts：（1）Bone marrow of 34 malignant lymphoma patients was examined by Flow Cytometry,23 cases were detected to have bone marrow involvement.（2）Among these 23 cases,19 cases were non-Hodgkin＇s lymphoma（NHL）,4 cases were Hodgkin＇s lymphoma（HL）.The highest frequency antigen marker of B cell NHL was CD19 and CD20,T cell NHL was CD7,and HL was CD9.Conclusions：（1）Flow Cytometry（CD45/SSC gate）is a feasible and effective method to detect patients with bone marrow involvement.（2）The antigen marker of Bcell NHL is CD19 and CD20,Tcell NHL is CD7 and HL is CD9.
出处
《中国癌症杂志》
CAS
CSCD
2005年第5期476-478,共3页
China Oncology
