摘要
为深入理解人类肾上腺(AD)的功能,构建了正常人肾上腺cDNA文库,并利用大规模表达序列标签(ESTs)测序和生物信息学技术,研究显示参与基因/蛋白表达的基因类型表达最多,其次为能量代谢类.肾上腺中表达丰度最高的3个基因均为参与类固醇合成的酶类和蛋白.一些重要的基因首次显示在肾上腺表达,包括神经激素和神经肽,如促肾上腺皮质激素释放激素(CRH),黑色素浓激素(MCH),urocortin,可卡因和安非他明调节肽(CART)和垂体腺苷酸环化酶激活肽P(ACAP);许多重要介质的受体,如细胞因子、神经肽及神经递质受体;参与胆固醇代谢的基因,如LDL受体、HDL结合蛋白和胆固醇合成酶.研究结果表明在肾上腺表达丰度最高的基因与该器官的功能特异性有关,除类固醇激素外,许多神经肽、细胞因子在肾上腺产生,肾上腺与体内其他重要的系统间存在广泛的应答,而且在人肾上腺局部可能存在一个CRH-ACTH-皮质醇调节网络.
In order to understand the functions of human adrenal glands （AD）, cDNA libraries of human normal adrenal were constructed. Large-scale sequencing expressed sequence tags （ESTs） and bioinformatics techniques were used. The categories of genes highly expressed in AD were gene/protein expression, followed by energy-metabolism. The first three genes highly expressed in AD were the enzymes and protein involved in the biosynthesis of steroids. It was first shown that some important genes were expressed in human adrenal,including hormones or secretory proteins, such as CRH, melanin concentration hormone （MCH）, urocortin,cocaine and amphetamine regulated transcript （CART）, and PACAP （pituitary adenylate cyclase activating polypeptide） were expressed in AD; many receptors, such as cytokine, neuropeptide, and neurotransmitter receptors, also existed in AD; cholesterol-metabolism-related genes, such as LDL receptor, HDL binding protein, cholesterol synthases. It suggests that the genes highly expressed in AD are closely related to the specific functions of adrenal. Besides steroids, many peptide hormones and cytokines are also expressed in AD. There is extensive cross-talking between adrenal and other systems by many important active peptides or cytokines. Furthermore, there may be a local CRH-ACTH-cortisol regulatory network in human adrenal.
出处
《生命科学研究》
CAS
CSCD
2005年第3期272-277,共6页
Life Science Research
基金
国家"863"计划项目(102-10-10-02)
国家自然科学基金资助项目(39970370)~~
关键词
人肾上腺
基因表达谱
表达序列标签
生物信息学
human adrenal gland
gene expression profile
expressed sequence tags（ESTs）
bioinformatics
