摘要
目的探讨三色荧光标记流式细胞术CD45辅助设门检测儿童急性白血病免疫表型的临床应用及意义。方法采用流式细胞仪和几组三色荧光标记抗体检测34例FAB分型为ALL与AML的儿童骨髓或外周血免疫表型。结果25例ALL儿童中,B型淋巴细胞白血病14例,T型淋巴细胞白血病6例,双标记5例,在T-ALL中,淋系抗原阳性率表达顺序为CD7>CD3>CD5,在B-ALL中,淋系抗原阳性率表达顺序为CD19>CD10>CD22>CD20,在T-ALL、B-ALL及双标记中还可同时表达髓系抗原(CD13、CD14、CD15、CD33)。9例AML儿童中,同时或分别表达髓系标志抗原CD15、CD33、CD13、CD14,其阳性表达率为89%、89%、79%、11%,9例中有3例还不同程度表达淋系抗原CD3、CD7、CD19。结论三色流式细胞术,可准确将白血病分为淋系与髓系白血病,并能将淋系白血病分为B型和T型及双标记,同时能反映髓系抗原阳性的ALL(My+ALL)与淋系抗原阳性的AML(AL+AML),对指导临床治疗有重要价值。
Objective To investigate the clinical significance of immunophenotye of children acute leukemia by 3 - color flow cytometry with CD45 gating. Methods lmmunophenotyes of bone marrow and peripheral blood samples from FAB- ALL and FAB- AML cells of 34 patients were analyzed by means of flow cytometry with 3 - color fluorescence labeling antibodies. Results Of the 25 patients with FAB - ALL cells, 14 were patients with B - ALL, 6 were patients with T - ALL and 5 were patients with double labeling. The results showed that the antigen expression frequencies were as follows： in T - ALL, CD7 〉 CD3 〉 CD5 and in B - ALL, CD19 〉 CD10 ） CD22 〉 CD20. Antigens such as CD13, CD14, CDI5, CD33 were expressed in all 3 groups and showed the same frequencies in B - ALL and T- ALL. Of the 9 patients with FAB - AML cells, the antigen expression frequencies were as follows： CD15（89% ）, CD33（89% ）, CD13（79% ） and CD14（ 11% ）. To different extent, 3 of 9 patients expressed antigen such as CD3, CD7 and CDI9. Conclusion Three - color flow cytometry can classify the patients into B - ALL, T - ALL and double - labeling. At the same time, it can express myeloid antigen ALL （My^＋ALL） and T antigen AML （AL^＋ AML）. This method is significant in guiding diagnosis and treatment.
出处
《宁夏医学院学报》
2005年第5期371-373,共3页
Journal of Ningxia Medical College
