摘要
目的:探讨新生猪缺氧缺血性脑损伤(HIBD)治疗时间窗的存在及其意义.方法:生后7天约克种白猪30头,随机分成对照组(5头)和实验组(25头).实验组分5组,每组5头,双侧颈总动脉结扎加4%低氧通气1h,分别于缺血缺氧(HI)后0、6、12、18和24h进行磁共振频谱分析(MRS),各时间点MR扫描后作脑组织热休克蛋白70(HSP 70)表达的免疫组化研究.结果:实验组HI后0h,Lac/NAA、Lac/Cho、Lac/Cr明显增高,NAA/Cho、NAA/Cr下降,未见明显HSP 70阳性表达细胞;HI后6～18h,NAA峰呈现进行性增高趋势,NAA/Cho、NAA/Cr比值升高,Lac峰呈进行性下降趋势,Lac/NAA、Lac/Cho、Lac/Cr降低,HSP 70阳性表达细胞进行性增加;HI后24h,NAA峰再次下降,NAA/Cho、NAA/Cr降低,Lac峰则再度明显升高,Lac/NAA、Lac/Cho、Lac/Cr均增高,HSP70阳性表达细胞明显减少;对照组各时间点MRS未见异常,脑组织未见HSP 70阳性表达细胞.结论:MRS可以比较准确地反映缺氧缺血性脑病的病理生理学演变过程,结合HSP 70的表达,在探讨HIE的治疗时间窗的存在及其意义上具有重要价值,在HIE的发生发展过程中确实存在潜在的治疗时间窗.
Objective： To discuss the existence and significance of therapeutic window in hypoxic-ischemic cerebral damage （HIBD） in piglets. Methods： Thirty 7-day-old piglets were divided into control and experimental groups. Piglets in experimental group were subjected to bilateral common carotid artery ligation followed by ventilation with low concentration oxygen （4%） for one hour. After the intervention, MR spectroscopy and the expression of heat shock protein 70 in brain tissues were evaluated. Results：In experimental group,NAA、Cho and Cr markedly decreased, Lac significantly increased at 0 hour post HI insult. From 6th hour to 18^th hour post HI insult,NAA and Lac progressively increased and decreased respectively. NAA and Lac decreased and increased again respectively at 24^th hour post HI insult. No positive HSP70-expressed cells were detected at 0 hour post HI insult. The number of positive HSP70-expressed cells progressively increased from 6th hour to 18^th hour post HI insult but markedly decreased at 24^th hour post HI insult. Conclusion： In combination with the expression of HSP70, MRS is a useful tool to evaluate hypoxic-ischemic encephalopathy. Therapeutic window truly exists in the process of occurrence and development in HIE.
出处
《放射学实践》
2005年第10期872-876,共5页
Radiologic Practice
关键词
缺氧缺血
脑
磁共振波谱学
新生猪
免疫化学
Hypoxia-ischemia, brain
Magnetic resonance spectroscopy
Piglet
Immunochemistry
