摘要
目的建立用HPLC测定大麦芽中麦黄酮的方法.方法采用色谱柱Spherigel C18(150 mm×4.6 mm,5μm),流动相为乙腈-水-四氢呋喃-甲酸(26:74:10:1),检测波长350 nm,体积流量1. 0 mL/min,进样量20 μL.结果麦黄酮在0.22～2.15 mg/L(r=0.999 8)峰面积与质量浓度呈良好的线性关系;平均回收率为97.4%,RSD为1.7%(n=9).结论该方法可用于大麦芽中麦黄酮的测定.
Objective To develop an HPLC method for the quantitative analysis of tricin in Methods HPLC method was used. Analysis was performed on Spherigel C18 column （150 mm×4.6 μm） with acetonitrile-water-tetrahydrofuran-methanoic acid （26 ： 74 ： 10 ： 1） as mobile phase. malt. mm, Thedetection wavelength was 350 nm, flow rate was 1.0 mL/min, and the analysic volumn was set at 20 μL. Results The calibration curve of tricin was linear in the range of 0.22-2.15 mg/L （r=0. 999 8）. The average recovery rate Of tricin was 97.4% （RSD=1. 7%） （n=9）. Conclusion The method can be used to determine tricin in raw malt quantitatively.
出处
《中草药》
CAS
CSCD
北大核心
2005年第11期1632-1634,共3页
Chinese Traditional and Herbal Drugs
基金
国家"十五"重大科技专项资助项目(2001BA701A55-50)
国家自然科学基金资助项目(30472190)
