摘要
采用电喷雾质谱法可同时检测到假单胞菌BS-03发酵液提取物中的鼠李糖脂及其自由酸前体(3-羟基葵酸单体和二聚体)。根据ESI-MS/MS的双鼠李糖脂二级质谱图显示存在强度较高的m/z为205、247的特征碎片离子峰,而单鼠李糖脂中却不存在此特征碎片离子。同时自由酸前体的主要组分为C8,C10,C8C10/C10C8,C10C10。其同分异构体与鼠李糖脂同分异构体类似,其中短链位于羟基端的组分含量高于长链在羟基端的组分。
A series of pseudomolecular and fragment ions attributed to 3-hydroxyalkanoic acids （HAs） and 3-（3-hydroxyalkanoyloxy） alkanoic acids （HAAs） were detected by ESI-MS among the rhamnolipids observed in Pseudomonas sp. BS-03 supernatant extracts. The structure of rhamnolipids, HAs and HAAs were deduced. According to ESI-MS/MS there were characteristic fragment ions at m/z 205, 247 in all dirhamnolipids while they did not exist in any monorhamnolipids. The most abundant free acid precursors were C8, C10, C8C10/C10C8 and C10 C10. Within an isomeric pair of HAAs, just like rhamnolipids the isomer with the shortest 3- hydroxyalkaloyl residues at the hydroxyl end was more abundant than the one with the heavier3-hydroxyalkaloyl acid at the same position.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2005年第10期83-87,共5页
China Biotechnology
基金
国家"十五"科技攻关资助项目(2001BA706B-18)
南京市科技发展计划资助项目(200301045)~~
