摘要
采用聚丙烯酰胺凝胶电泳和免疫印迹法分析不育症病人抗精浆免疫抑制物抗体(SPIM-Ab)对特异抗原的免疫反应,并与ELISA法进行比较。结果表明,免疫印迹法检测血清、清浆SPIM-Ab阳性率分别为30.3%(32/107)和29.0%(36/124),ELISA法检测分别为33.6%(36/107)和31.5%(39/124),X2=129.58,P<0.01.免疫印迹法分析的68份不育症病人的血清、精浆SPIM-Ab阳性样本中,49份(72.1%)与特异抗原的分子量110000和(或)94000区带发生免疫反应,19份(27.9%)与分子量52000区带反应,13份(19.1%)与分子量36000区带反应。提示大分子量SPIM抗原可能更容易诱导高效价SPIM-Ab产生,并形成抗原抗体免疫复合物干扰生育。
The immune reactions of anti-seminal plasma immuno-inhibition material antibodies (anti-SPIM-Ab) with specific antigens in infertile patients were analyzed by SDS-PAGE and Western blotting, and the results were compared with those determined by ELISA. It was showed that the positive rates of anti-SPIM-Ab determined by Western blotting in sera and seminal plasma from infertile patients were 30. 0% (32/107) and 29. 0% (36/124), while 33. 6% (36/107) and 31. 5% (39/124) by ELISA respectively (X2=129. 58, P<0. 01). Among 68 samples with positive anti-SPIM-Ab in both sera and seminal plasma 49 (72. 1%) of samples reacted to the 110 kD and/or 94 kD bands of SPIM specific antigens, but 19(27. 9%) and 13 (19. 1%) of samples reacted with 52 kD and 36 kD band respectively. It is suggested that the SPIM antigens with high molecular weight may easily induce hith titer anti-SPIM antibodies, produce their immune complexes and interfere with fertility.