摘要
基于传统的平行平板流室理论和方法,设计制作一套可用于破骨细胞等细胞经受流体剪应力作用的实验系统。用金属夹具和医用硅橡胶密封圈封闭流室,同时不影响流室高度的准确性。调整载玻片与上储液池液面到同一水平面,降低了液体静压力对细胞的影响。此系统可用于研究破骨细胞等细胞在形态学、生理生化等方面对剪应力的反应。
On the basis of the well-established principles and techniques about flow chamber, we have designed and made a kind of parallel plate system to apply steady fluid shear stress to osteoclast-like cells etc. in vitro. Biocompatible rubber and metal clamping apparatus made of aluminium alloy are used to seal the flow chamber without changing its even height designed beforehand. The influence of hydrostatic pressure on cells is minimized by adjusting the glass slide and the fluid surface in the upper reservoir to the same horizontal plane. This system can be used to investigate the responses of osteoclast-like cells etc. to fluid shear force in terms of morphology,physiology or biochemistry.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2005年第2期288-292,共5页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目 (3 0 2 714 2 8)
教育部"优秀青年教师资助计划"项目 (教人司 [2 0 0 2 ] 40号 )
高等学校博士学科点专项科研基金资助项目 (B12 0 0 0 5 8)
