摘要
目的观察Ⅰ型胶原涂层的聚羟基丁酸羟基戊酸酯(PHBV)对半月板纤维软骨细胞的吸附作用及对细胞生物学性状的影响,评价其作为半月板组织工程支架的可行性及价值。方法构建Ⅰ型胶原涂层的PHBV半月板假体,将体外培养的兔半月板纤维软骨细胞吸附于该支架上三维立体培养,通过相差倒置显微镜、组织学、扫描电镜及免疫组织化学检测支架对半月板纤维软骨细胞的表型、增殖及功能的影响。结果PHBV能制成具有一定力学强度的半月板假体,通过Ⅰ型胶原涂层后增强了其细胞吸附能力,半月板纤维软骨细胞在支架孔壁贴附良好,维持表型稳定,分泌胞外基质。结论Ⅰ型胶原涂层的PHBV支架细胞相容性良好,能按半月板的大小、形状制成有一定力学强度的半月板假体,是比较理想的半月板组织工程支架材料。
Objective To evaluate the feasibility and value of collagen coated Poly-β-hydroxybutyrate-co-β-hydroxyvalerate （PHBV） as scaffold for meniscus tissue engineering after observing how it absorbs meniscal fibrochondrocytes and affects cell＇s character. Methods The artificial meniscal prostheses were constructed with typeⅠ collagen coated PHBV. Meniscal fibrochondrocytes were isolated and multiplied in vitro, then fibrochondrocytes were seeded onto porous collagen coated PHBV scaffold and cultured in a three dimensional environment. The effects of the scaffold on cell adhesion, proliferation, morphological changes, and synthesis of the extracellular matrix were observed by phase-contrast microscopy, histological method, scanning electron microscopy and immunohistochemistry. Results The artificial meniscal prostheses could be constructed with good mechanical property with PHBV. Its absorbability to cell was enhanced after coated with type Ⅰ collagen. Meniscal fibrochondrocytes attached to wall of microholes of the scaffold had largely maintained a rounded morphology and could secrete the extracellular matrix on the porous scaffold. Conclusion Type Ⅰ collagen coated PHBV scaffold has good cellular compatibility. It can be used to construct artificial meniscal prostheses with good mechanical property according to meniscal size and shape. It is an ideal scaffold for building tissue engineered meniscus.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第11期1390-1392,共3页
Chinese Journal of Experimental Surgery
基金
广州市科技攻关重大项目(2002Z1-E0034)
广东省科技计划资助项目(2003A3020102)
