摘要
目的探讨产甲酸草酸杆菌分离培养及鉴定方法.方法应用选择性培养基,在37 ℃厌氧环境下分离培养人肠道产甲酸草酸杆菌.通过观察菌落形态、细菌涂片染色镜检、离子色谱仪及核酸序列分析,对细菌进行鉴定.结果产甲酸草酸杆菌菌落形态为白色点状或梭状,大小约(0.1～0.5) mm×(1.0～3.0) mm;菌落周边出现直径约3.5～4.5 mm的清晰透明圈.该细菌为革兰氏阴性杆菌,大小约(1.0～1.6) μm×(3.0～6.5) μm.液体培养基中草酸浓度随细菌浓度增加而逐渐下降.与文献报道核酸序列比较,该细菌分解草酸的功能基因frc、oxc的核酸同源性分别为95.8%及93.6%.结论产甲酸草酸杆菌可通过选择性培养基、在厌氧环境下从人粪便中分离出来.
Objective To study the method of isolation and identification of the Oxalobacter formigenes from the intestines of human. Methods The Oxalobaeter formigenes were isolated from human fecal specimens and cultivated under anaerobic conditions at 37℃ in medium OxB and OxD. The identification of Oxalobaeter formigene strains was performed by stereoscopic microscope, chromatography and sequence comparison, Results The typical colonies were white dot or fusiform and differed from sizes （0.1-0.5） mm×（1.0-3.0） ram. The colony was surrounded by a clear zone with the maximum diameter of 4.5 mm. The Oxalobaeter formigenes were Gram-negative in size of （1.0-1.6） μm×（3.0-6.5）μm. It has been shown that the concentration of oxalate in OxB medium gradually decreased along with the increase of Oxalobaeter formigenes. Comparing the nueleie acid of fro and oxe gene in our research with those of reported by gene bank, the homology of nueleotide was 95,8% and 93.6% respectively. Conclusion The Oxalobaeter formigenes can be isolated from the intestines of human and cultivated under anaerobic conditions successfully.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第11期1402-1403,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目(30371423)
